@article{71cc446901084e688e3271272ee9ad07,
title = "Inhibition of MT1-MMP activity using functional antibody fragments selected against its hemopexin domain",
abstract = "The membrane associated MMP, MT1-MMP, is a critical pericellular protease involved in tumour cell invasion and angiogenesis and is highly up-regulated in numerous human cancers. It therefore represents an exciting new therapeutic cancer-specific target. We have generated recombinant human scFv antibodies against the non-catalytic, hemopexin domain of MT1-MMP that modulate its interactions with collagen. One of these is an effective inhibitor of the invasive capacity of cancer cells and of angiogenesis in model systems. This demonstrates that targeting sites outside the catalytic domain presents a potential novel approach to proteinase inhibition that could have applications in cancer therapeutics.",
keywords = "Angiogenesis, Collagenolysis, Hemopexin, MT1-MMP, scFv",
author = "B. Basu and {Correa De Sampaio}, P. and H. Mohammed and M. Fogarasi and P. Corrie and Watkins, {N. A.} and Smethurst, {P. A.} and English, {W. R.} and Ouwehand, {W. H.} and G. Murphy",
note = "Funding Information: The technical advice of the following people during this project is gratefully acknowledged: Dr. Nicola Foad for the CaM-tagged antigen production; Dr. James Allpress (MedImmune) for the isolation and screening of antibodies by phage display; Drs Susan Atkinson and Dr. Christian Roghi for advice on the biochemical and cell biological assays; Dr. Neil Taylor for sequence alignments, the Cambridge Research Institute Biorepository team for help with Incucyte{\texttrademark} assays. The V gene library was kindly supplied by MedImmune, formerly Cambridge Antibody Technology (Cambridge). This project was funded by a Cancer Research UK Gordon Hamilton-Fairley Clinical Research Training Fellowship (BB), Cancer Research UK (GM, MF, WRE), Hutchison Whampoa (GM), Portugese Foundation for Science and Technology (PCS) and the Addenbrooke's Charitable Trust (HM). WHO, NAW and PAS are supported by a programme grant (No. RP-PG-0310-1002) from the National Institute for Health Research in England to NHS Blood and Transplant. ",
year = "2012",
month = feb,
doi = "10.1016/j.biocel.2011.11.015",
language = "English (US)",
volume = "44",
pages = "393--403",
journal = "International Journal of Biochemistry and Cell Biology",
issn = "1357-2725",
publisher = "Elsevier Limited",
number = "2",
}