InPouch TV™ culture for detection of Trichomonas vaginalis

Seema Sood, Srujana Mohanty, Arti Kapil, Jorge Tolosa, Suneeta Mittal

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Background & objectives: Trichomonas vaginalis accounts for almost half of all curable sexually transmitted infections and has also been associated with adverse outcomes of pregnancy and increased risk of HIV in women. Diagnosis of the condition by direct wet mount examination has a low sensitivity. Herein, we describe our experience with InPouch culture system for the detection of T. vaginalis. Methods: This prospective study was carried out from May 2003 to April 2004 among women presenting with genitourinary symptoms attending a primary health center clinic in Ballabhgarh, India. Two vaginal swabs (cotton tips) were obtained from each woman. The first swab was obtained from the lateral wall of vagina and was used to make a wet mount preparation. The second swab was obtained from the posterior fornix of the vagina and inoculated in the InPouch for culture of T. vaginalis. Results: Of the 601 women, 22 were positive by direct microscopy for T. vaginalis while 40 were positive by culture. Overall, T. vaginalis accounted for 6.7 per cent of reproductive tract infections. Interpretation & conclusion: The InPouch TV culture system is a simple, cost-effective and a sensitive method for diagnosing T. vaginalis and may be recommended for routine use in diagnosing genital tract infections.

Original languageEnglish (US)
Pages (from-to)567-571
Number of pages5
JournalIndian Journal of Medical Research
Issue number4
StatePublished - Apr 2007


  • InPouch culture
  • Reproductive tract infections
  • Sexually transmitted infections
  • Trichomonas vaginalis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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