@article{ecca0d3eb6d145aabadaedfc83d49a42,
title = "Interaction landscape of membrane-protein complexes in Saccharomyces cerevisiae",
abstract = "Macromolecular assemblies involving membrane proteins (MPs) serve vital biological roles and are prime drug targets in a variety of diseases. Large-scale affinity purification studies of soluble-protein complexes have been accomplished for diverse model organisms, but no global characterization of MP-complex membership has been described so far. Here we report a complete survey of 1,590 putative integral, peripheral and lipid-anchored MPs from Saccharomyces cerevisiae, which were affinity purified in the presence of non-denaturing detergents. The identities of the co-purifying proteins were determined by tandem mass spectrometry and subsequently used to derive a high-confidence physical interaction map encompassing 1,726 membrane protein-protein interactions and 501 putative heteromeric complexes associated with the various cellular membrane systems. Our analysis reveals unexpected physical associations underlying the membrane biology of eukaryotes and delineates the global topological landscape of the membrane interactome.",
author = "Mohan Babu and James Vlasblom and Shuye Pu and Xinghua Guo and Chris Graham and Bean, {Bj{\"o}rn D.M.} and Burston, {Helen E.} and Vizeacoumar, {Franco J.} and Jamie Snider and Sadhna Phanse and Vincent Fong and Tam, {Yuen Yi C.} and Michael Davey and Olha Hnatshak and Navgeet Bajaj and Shamanta Chandran and Thanuja Punna and Constantine Christopolous and Victoria Wong and Analyn Yu and Gouqing Zhong and Joyce Li and Igor Stagljar and Elizabeth Conibear and Wodak, {Shoshana J.} and Andrew Emili and Greenblatt, {Jack F.}",
note = "Funding Information: Acknowledgements We thank H. Riezman, K. Sato and A. Nakano for providing strains and reagents, C. Ungermann for Sec18 antisera, A. Pierleoni for assistance with MemPype and R. Zheng for technical assistance. This research was supported by grants from the Canadian Foundation for Innovation, the Canadian Institutes of Health Research (CIHR MOP no. 81156, MOP no. 64394 and MOP no. 82940), the Canadian Cancer Society Research Institute, the Heart and Stroke Foundation, the Cystic Fibrosis Foundation, Novartis, the Ontario Genomics Institute and Genome Canada. E.C. is a CIHR New Investigator. S.J.W. is a Canada Research Chair Tier-I and acknowledges support from the SickKids Foundation.",
year = "2012",
month = sep,
day = "27",
doi = "10.1038/nature11354",
language = "English (US)",
volume = "489",
pages = "585--589",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "7417",
}