TY - JOUR
T1 - Interaction of AbrB, a transcriptional regulator from Bacillus subtilis with the promoters of the transition state-activated genes tycA and spoVG
AU - Fürbaß, Rainer
AU - Gocht, Martin
AU - Zuber, Peter
AU - Marahiel, Mohamed A.
PY - 1991/3
Y1 - 1991/3
N2 - In Bacillus subtilis the abrB gene product negatively affects the transcription of some genes activated during the transition from vegetative to stationary phase of growth. Interaction of AbrB with the promoters of two such genes, spoVG, a sporulation gene, and tycA, an antibiotic biosynthesis gene, was studied by DNase I and hydroxyl radical footprinting. Two binding areas within the leader and promoter regions of tycA were identified. In spoVG the binding site is located at the A+T-rich region upstream of the promoter. Hydroxyl radical footprinting revealed that the AbrB-protected regions, in both the tycA and spoVG promoters, are short A+T-rich regions that are separated by one helical turn, indicating that AbrB binds to one face of the helix. To examine the role of spoOA in the expression of abrB-controlled genes, the levels of AbrB protein in Spo+ and in spoOA cells were determined by Western blot analysis. In wild-type cells AbrB was detected only during vegetative growth, whereas in spoOA cells a high level of AbrB was detected during both the vegetative and stationary phases of growth. These findings support a model in which (i) spoOA negatively affects abrB expression, and (ii) the repression of the transition state-activated genes tycA and spoVG in spoOA cells is due to constitutive expression of AbrB, which acts as a repressor.
AB - In Bacillus subtilis the abrB gene product negatively affects the transcription of some genes activated during the transition from vegetative to stationary phase of growth. Interaction of AbrB with the promoters of two such genes, spoVG, a sporulation gene, and tycA, an antibiotic biosynthesis gene, was studied by DNase I and hydroxyl radical footprinting. Two binding areas within the leader and promoter regions of tycA were identified. In spoVG the binding site is located at the A+T-rich region upstream of the promoter. Hydroxyl radical footprinting revealed that the AbrB-protected regions, in both the tycA and spoVG promoters, are short A+T-rich regions that are separated by one helical turn, indicating that AbrB binds to one face of the helix. To examine the role of spoOA in the expression of abrB-controlled genes, the levels of AbrB protein in Spo+ and in spoOA cells were determined by Western blot analysis. In wild-type cells AbrB was detected only during vegetative growth, whereas in spoOA cells a high level of AbrB was detected during both the vegetative and stationary phases of growth. These findings support a model in which (i) spoOA negatively affects abrB expression, and (ii) the repression of the transition state-activated genes tycA and spoVG in spoOA cells is due to constitutive expression of AbrB, which acts as a repressor.
KW - AbrB protein-DNA interaction
KW - Bacillus subtilis
KW - Hydroxyl radical footprint
KW - spoVG
KW - tycA
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U2 - 10.1007/BF00261673
DO - 10.1007/BF00261673
M3 - Article
C2 - 1850083
AN - SCOPUS:0026019195
SN - 0026-8925
VL - 225
SP - 347
EP - 354
JO - MGG Molecular & General Genetics
JF - MGG Molecular & General Genetics
IS - 3
ER -