Isohormones of chorionic gonadotropin produced by cloned cell lines in culture

J. M. Hammond, W. E. Bridson, A. Chrambach, P. O. Kohler

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


Chorionic gonadotropin (CG) synthesized by human choriocarcinoma cells in culture was fractionated by isoelectric focusing on polyacrylamide gel and examined for biological (B) and immunological (I) activity. Two isohormones of CG with isoelectric points (pI’s) of 3.0 and 4.5 were found in the culture medium from each of three clonal lines studied. The isohormones of CG with pi 3.0 had a B/I ratio of 2.5 while the isohormone with pi 4.5 had a B/I ratio of 0.5. The relative proportions of these two isohormones correlated with the B/I ratios previously found for the unfractionated CG from the same clones.

Original languageEnglish (US)
Pages (from-to)185-187
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Issue number1
StatePublished - 1972
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical


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