TY - JOUR
T1 - Label-free in vivo optical imaging of functional microcirculations within meninges and cortex in mice
AU - Jia, Yali
AU - Wang, Ruikang K.
N1 - Funding Information:
This work was supported in part by research grants from the National Heart, Lung, and Blood Institute ( R01 HL093140 ); National Institute of Biomedical Imaging and Bioengineering ( R01 EB009682 ); National Institute of Deafness and other Communication Disorders ( R01 DC010201 ); and the American Heart Association ( 0855733G ).
PY - 2010/12/15
Y1 - 2010/12/15
N2 - Abnormal microcirculation within meninges is common in many neurological diseases. There is a need for an imaging method that is capable of monitoring dynamic meningeal microcirculations, preferably decoupled from cortical blood flow. Optical microangiography (OMAG) is a recently developed label-free imaging method capable of producing 3D images of dynamic blood perfusion within micro-circulatory tissue beds at an imaging depth up to ∼2 mm, with an unprecedented imaging sensitivity to blood flow at ∼4 μm/s. In this paper, we demonstrate the utility of OMAG in imaging the detailed blood flow distributions, at a capillary level resolution, within the meninges and cortex in mice with the cranium left intact. Using a thrombotic mouse model, we show that the OMAG can yield longitudinal measurements of meningeal vascular responses to the insult and can decouple these responses from those in the cortex, giving valuable information regarding the localized hemodynamics along with the dynamic formation of thrombotic event. The results indicate that OMAG can be a useful tool to study therapeutic strategies in preclinical animal models in order to mitigate various pathologies that are mainly related to the meningeal circulations.
AB - Abnormal microcirculation within meninges is common in many neurological diseases. There is a need for an imaging method that is capable of monitoring dynamic meningeal microcirculations, preferably decoupled from cortical blood flow. Optical microangiography (OMAG) is a recently developed label-free imaging method capable of producing 3D images of dynamic blood perfusion within micro-circulatory tissue beds at an imaging depth up to ∼2 mm, with an unprecedented imaging sensitivity to blood flow at ∼4 μm/s. In this paper, we demonstrate the utility of OMAG in imaging the detailed blood flow distributions, at a capillary level resolution, within the meninges and cortex in mice with the cranium left intact. Using a thrombotic mouse model, we show that the OMAG can yield longitudinal measurements of meningeal vascular responses to the insult and can decouple these responses from those in the cortex, giving valuable information regarding the localized hemodynamics along with the dynamic formation of thrombotic event. The results indicate that OMAG can be a useful tool to study therapeutic strategies in preclinical animal models in order to mitigate various pathologies that are mainly related to the meningeal circulations.
KW - Blood flow
KW - Meningeal blood vessel network
KW - Microcirculation
KW - Optical microangiography
KW - Thrombosis
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U2 - 10.1016/j.jneumeth.2010.09.021
DO - 10.1016/j.jneumeth.2010.09.021
M3 - Article
C2 - 20933005
AN - SCOPUS:78649522721
SN - 0165-0270
VL - 194
SP - 108
EP - 115
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 1
ER -