Lectin-induced regulation of human thymus-independent anti-TNP responses in vitro

B. Golding, D. R. Burger, M. B. Rittenberg

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3 Scopus citations


Recently we showed that human hapten-specific T cell-independent (TI) antibody responses could be elicited in vitro with the antigen trinitrophenylated Brucella abortus (TNP-Ba). Although by definition T cells are not required for TI responses, they have been shown capable of regulating such responses in the mouse. In this study we examine the ability of the T cell lectins Con A and PHA to modulate TI responses of human tonsil cells. Addition of the lectins to cultures on day 0 or 3 resulted in inhibition (> 90%) or enhancement (> 150%) of the anti-TNP PFC response, respectively. The inhibition was only apparent if the lectins were added at the onset of culture but not 24 hr later, and was T cell-dependent, because T cell-depleted cultures (< 0.5% E-rosetting cells) could not be inhibited. The enhancement observed was even greater in T cell-depleted cultures and was antigen-specific because TNP-SRBC but not PC-SRBC targets were lysed and the PFC were inhibited by soluble TNP (> 70%). This finding suggested that anti-TNP PFC precursors were proliferating more vigorously or that additional, previously antigen-insensitive cells were being recruited in the presence of lectin.

Original languageEnglish (US)
Pages (from-to)942-945
Number of pages4
JournalJournal of Immunology
Issue number3
StatePublished - 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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