Ligand-induced allostery in the interaction of the Pseudomonas aeruginosa heme binding protein with heme oxygenase

Daniel J. Deredge, Weiliang Huang, Colleen Hui, Hirotoshi Matsumura, Zhi Yue, Pierre Moënne-Loccoz, Jana Shen, Patrick L. Wintrode, Angela Wilks

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


A heme-dependent conformational rearrangement of the C-Terminal domain of heme binding protein (PhuS) is required for interaction with the iron-regulated heme oxygenase (HemO). Herein, we further investigate the underlying mechanism of this conformational rearrangement and its implications for heme transfer via site-directed mutagenesis, resonance Raman (RR), hydrogen- deuterium exchange MS (HDX-MS) methods, and molecular dynamics (MD). HDX-MS revealed that the apo-PhuS C-Terminal α6/α7/α8-helices are largely unstructured, whereas the apo-PhuS H212R variant showed an increase in structure within these regions. The increased rate of heme association with apo-PhuS H212R compared with the WT and lack of a detectable five-coordinate highspin (5cHS) heme intermediate are consistent with a more folded and less dynamic C-Terminal domain. HDX-MS and MD of holo-PhuS indicate an overall reduction in molecular flexibility throughout the protein, with significant structural rearrangement and protection of the heme binding pocket.We observed slow cooperative unfolding/ folding events within the C-Terminal helices of holo-PhuS and the N-Terminal α1/α2-helices that are dampened or eliminated in the holo-PhuS H212R variant. Chemical cross-linking and MALDI-TOF MS mapped these same regions to the PhuS:HemO protein-protein interface. We previously proposed that the protein-protein interaction induces conformational rearrangement, promoting a ligand switch from His-209 to His-212 and triggering heme release to HemO. The reduced conformational freedom of holo-PhuS H212R combined with the increase in entropy and decrease in heme transfer on interaction with HemO further support this model. This study provides significant insight into the role of protein dynamics in heme binding and release in bacterial heme transport proteins.

Original languageEnglish (US)
Pages (from-to)3421-3426
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number13
StatePublished - Mar 28 2017


  • Allostery
  • Heme Binding
  • Heme Transfer
  • Protein-Protein Interaction
  • Pseudomonas Aeruginosa

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Ligand-induced allostery in the interaction of the Pseudomonas aeruginosa heme binding protein with heme oxygenase'. Together they form a unique fingerprint.

Cite this