Light-sheet fluorescence microscopy for the study of the murine heart

Yichen Ding; Zachary Bailey; Victoria Messerschmidt; Jun Nie; Richard Bryant; Sandra Rugonyi; Peng Fei; Juhyun Lee; Tzung K. Hsiai

doi:10.3791/57769

Light-sheet fluorescence microscopy for the study of the murine heart

Yichen Ding, Zachary Bailey, Victoria Messerschmidt, Jun Nie, Richard Bryant, Sandra Rugonyi, Peng Fei, Juhyun Lee, Tzung K. Hsiai

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain stripes or artifacts and suffer from a reduced resolution due to the scattering and absorption of light by the tissue. This study uses a dual-sided illumination beam and a simplified CLARITY optical clearing technique for the murine heart. These techniques allow for deeper imaging by removing lipids from the heart and produce a large field of imaging, greater than 10 x 10 x 10 mm³. As a result, this strategy enables us to quantify the ventricular dimensions, track the cardiac lineage, and localize the spatial distribution of cardiac-specific proteins and ion-channels from the post-natal to adult mouse hearts with sufficient contrast and resolution.

Original language	English (US)
Article number	e57769
Journal	Journal of Visualized Experiments
Volume	2018
Issue number	139
DOIs	https://doi.org/10.3791/57769
State	Published - Sep 15 2018

Keywords

Bioengineering
CLARITY
Cardiomyocytes
Dual illumination
Heart
Issue 139
LSFM
Light sheet fluorescence microscopy
Murine
Optical clearing
Ventricle

ASJC Scopus subject areas

Neuroscience(all)
Chemical Engineering(all)
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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title = "Light-sheet fluorescence microscopy for the study of the murine heart",

abstract = "Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain stripes or artifacts and suffer from a reduced resolution due to the scattering and absorption of light by the tissue. This study uses a dual-sided illumination beam and a simplified CLARITY optical clearing technique for the murine heart. These techniques allow for deeper imaging by removing lipids from the heart and produce a large field of imaging, greater than 10 x 10 x 10 mm3. As a result, this strategy enables us to quantify the ventricular dimensions, track the cardiac lineage, and localize the spatial distribution of cardiac-specific proteins and ion-channels from the post-natal to adult mouse hearts with sufficient contrast and resolution.",

keywords = "Bioengineering, CLARITY, Cardiomyocytes, Dual illumination, Heart, Issue 139, LSFM, Light sheet fluorescence microscopy, Murine, Optical clearing, Ventricle",

author = "Yichen Ding and Zachary Bailey and Victoria Messerschmidt and Jun Nie and Richard Bryant and Sandra Rugonyi and Peng Fei and Juhyun Lee and Hsiai, {Tzung K.}",

note = "Funding Information: The authors would like to express gratitude to Thao Nguyen and Atsushi Nakano from UCLA for providing the mice sample to image. This study was supported by grants NIH HL118650 (to Tzung K. Hsiai), HL083015 (to Tzung K. Hsiai), HD069305 (to N. C. Chi and Tzung K. Hsiai.), HL111437 (to Tzung K. Hsiai and N. C. Chi), HL129727 (to Tzung K. Hsiai), and University of Texas System STARS funding (to Juhyun Lee). Publisher Copyright: {\textcopyright} 2018, Journal of Visualized Experiments. All rights reserved.",

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AU - Ding, Yichen

AU - Bailey, Zachary

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AU - Bryant, Richard

AU - Rugonyi, Sandra

AU - Fei, Peng

AU - Lee, Juhyun

AU - Hsiai, Tzung K.

N1 - Funding Information: The authors would like to express gratitude to Thao Nguyen and Atsushi Nakano from UCLA for providing the mice sample to image. This study was supported by grants NIH HL118650 (to Tzung K. Hsiai), HL083015 (to Tzung K. Hsiai), HD069305 (to N. C. Chi and Tzung K. Hsiai.), HL111437 (to Tzung K. Hsiai and N. C. Chi), HL129727 (to Tzung K. Hsiai), and University of Texas System STARS funding (to Juhyun Lee). Publisher Copyright: © 2018, Journal of Visualized Experiments. All rights reserved.

PY - 2018/9/15

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N2 - Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain stripes or artifacts and suffer from a reduced resolution due to the scattering and absorption of light by the tissue. This study uses a dual-sided illumination beam and a simplified CLARITY optical clearing technique for the murine heart. These techniques allow for deeper imaging by removing lipids from the heart and produce a large field of imaging, greater than 10 x 10 x 10 mm3. As a result, this strategy enables us to quantify the ventricular dimensions, track the cardiac lineage, and localize the spatial distribution of cardiac-specific proteins and ion-channels from the post-natal to adult mouse hearts with sufficient contrast and resolution.

AB - Light-sheet fluorescence microscopy has been widely used for rapid image acquisition with a high axial resolution from micrometer to millimeter scale. Traditional light-sheet techniques involve the use of a single illumination beam directed orthogonally at sample tissue. Images of large samples that are produced using a single illumination beam contain stripes or artifacts and suffer from a reduced resolution due to the scattering and absorption of light by the tissue. This study uses a dual-sided illumination beam and a simplified CLARITY optical clearing technique for the murine heart. These techniques allow for deeper imaging by removing lipids from the heart and produce a large field of imaging, greater than 10 x 10 x 10 mm3. As a result, this strategy enables us to quantify the ventricular dimensions, track the cardiac lineage, and localize the spatial distribution of cardiac-specific proteins and ion-channels from the post-natal to adult mouse hearts with sufficient contrast and resolution.

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Light-sheet fluorescence microscopy for the study of the murine heart

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