TY - JOUR
T1 - Localisation of interleukin 8 in the synovial membrane, cartilagepannus junction and chondrocytes in rheumatoid arthritis
AU - Deleuran, B.
AU - Lemche, P.
AU - Kristensen, M.
AU - Chu, C. Q.
AU - Field, M.
AU - Jensen, J.
AU - Matsushima, K.
AU - Stengaard-Pedersen, K.
N1 - Funding Information:
This work was supported by grants from Danish Rheumatism Association. Dr Chu and Dr Field were supported by the Arthritis and Rheumatism Council of Britain. The authors would like to thank Dr M. Bayliss for providing normal joint tissue samples and Susanne Lemche for skilful technical assistance.
PY - 1994
Y1 - 1994
N2 - Interleukin-8 (IL-8) may play an important role in the development of synovitis in rheumatoid arthritis (RA), in that it is a powerful chemoattractant for neutrophils and T cells. The aim of this study was to examine the distribution of IL-8 in the synovial membrane and cartilage, from RA, osteoarthritis (OA) and normal joints. By immunohistochemical techniques, IL-8 was shown to be present in the lining layer cells in RA (87% and in OA (62% By contrast, only a few of the normal synovial lining layer cells (14% contained IL-8. Deeper in the membrane the number of IL-8 positive cells decreased. Only vessels were highly positive for IL-8. At the RA cartilage-pannus junction 26% of the cells contained IL-8, whereas at the OA cartilage-pannus junction 8% of the cells were IL-8 positive (P < 0.05). Chondrocytes present in joint surface cartilage stained positive for IL-8 in an average of 20% of the cells of both RA and OA. These results provide histological evidence that IL-8 is present in the arthritic synovial tissue and cartilage, and is distributed in a manner that may form a chemotactic gradient, which favours localisation of neutrophils to the joint lumen.
AB - Interleukin-8 (IL-8) may play an important role in the development of synovitis in rheumatoid arthritis (RA), in that it is a powerful chemoattractant for neutrophils and T cells. The aim of this study was to examine the distribution of IL-8 in the synovial membrane and cartilage, from RA, osteoarthritis (OA) and normal joints. By immunohistochemical techniques, IL-8 was shown to be present in the lining layer cells in RA (87% and in OA (62% By contrast, only a few of the normal synovial lining layer cells (14% contained IL-8. Deeper in the membrane the number of IL-8 positive cells decreased. Only vessels were highly positive for IL-8. At the RA cartilage-pannus junction 26% of the cells contained IL-8, whereas at the OA cartilage-pannus junction 8% of the cells were IL-8 positive (P < 0.05). Chondrocytes present in joint surface cartilage stained positive for IL-8 in an average of 20% of the cells of both RA and OA. These results provide histological evidence that IL-8 is present in the arthritic synovial tissue and cartilage, and is distributed in a manner that may form a chemotactic gradient, which favours localisation of neutrophils to the joint lumen.
KW - Cartilage-pannus junction
KW - Chondrocytes
KW - Interleukin 8
KW - Rheumatoid arthritis
KW - Synovial membrane
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U2 - 10.3109/03009749409102126
DO - 10.3109/03009749409102126
M3 - Article
C2 - 8108662
AN - SCOPUS:0028009807
SN - 0300-9742
VL - 23
SP - 2
EP - 7
JO - Scandinavian Journal of Rheumatology
JF - Scandinavian Journal of Rheumatology
IS - 1
ER -