Mammalian cloning: Possibilities and threats

S. M. Mitalipov, D. P. Wolf

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The cloning of mammals originated with the production of limited numbers of genetically identical offspring by blastomere separation or embryo splitting. In the past few years, remarkable progress has been reported in cloning by nuclear transfer (NT) with donor nuclei recovered from embryonic, fetal or adult cells. Factors that contribute to the successful reprogramming of the transferred nucleus and the normal term development of the newly reconstructed embryo include the cell cycle stage of both the donor nucleus and recipient cytoplast, the timing of fusion and cytoplast activation, and the source of donor nuclei. The possibility of producing live offspring by somatic cell NT carries potential applications in animal husbandry, biotechnology, transgenic and pharmaceutical production, biomedical research, and the preservation of endangered species. However, the low efficiencies of cloning by NT coupled with high embryonic, fetal and neonatal losses may restrict immediate commercial applications in agriculture. These limitations notwithstanding, the greatest benefits and practical implications of this new technology could be in transplantation medicine and therapeutic cloning.

Original languageEnglish (US)
Pages (from-to)462-468
Number of pages7
JournalAnnals of Medicine
Issue number7
StatePublished - 2000


  • Cloning
  • Nuclear reprogramming
  • Nuclear transfer

ASJC Scopus subject areas

  • General Medicine


Dive into the research topics of 'Mammalian cloning: Possibilities and threats'. Together they form a unique fingerprint.

Cite this