Mapping of a putative genetic locus determining ethanol intake in the mouse

D. Goldman; R. G. Lister; J. C. Crabbe

doi:10.1016/0006-8993(87)91241-8

Mapping of a putative genetic locus determining ethanol intake in the mouse

D. Goldman, R. G. Lister, J. C. Crabbe

Behavioral Neuroscience

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

Original language	English (US)
Pages (from-to)	220-226
Number of pages	7
Journal	Brain research
Volume	420
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(87)91241-8
State	Published - Sep 15 1987

Keywords

Alcohol
Ethanol acceptance
Inbred strain
LTW-4
Mouse
Pharmacogenetics
Protein polymorphism
Recombinant inbred strain
Two-dimensional electrophoresis

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(87)91241-8

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title = "Mapping of a putative genetic locus determining ethanol intake in the mouse",

abstract = "In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.",

keywords = "Alcohol, Ethanol acceptance, Inbred strain, LTW-4, Mouse, Pharmacogenetics, Protein polymorphism, Recombinant inbred strain, Two-dimensional electrophoresis",

author = "D. Goldman and Lister, {R. G.} and Crabbe, {J. C.}",

note = "Funding Information: These experiments were partially supported by grants from the Veterans Administration and by PHS-ADAMHA Grants AA05828, AA06243 and AA06498. We thank L.A. Akhtar, E.R. Young, B.R. Tam and A. Kosobud for assistance in collecting these data.",

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doi = "10.1016/0006-8993(87)91241-8",

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AU - Lister, R. G.

AU - Crabbe, J. C.

N1 - Funding Information: These experiments were partially supported by grants from the Veterans Administration and by PHS-ADAMHA Grants AA05828, AA06243 and AA06498. We thank L.A. Akhtar, E.R. Young, B.R. Tam and A. Kosobud for assistance in collecting these data.

PY - 1987/9/15

Y1 - 1987/9/15

N2 - In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

AB - In the mouse, there is evidence that a single genetic locus is a major determinant of differences in ethanol intake between some preferring and non-preferring inbred strains. In this report, we present evidence from two independent experiments indicating that this locus maps to chromosome 1 and that its expressed product is the abundant protein LTW-4 (a 28 kDa, pI 5.6 protein expressed in brain, liver and kidney). The genetic association was found using a panel of 14 polypeptides of mouse brain which were visualized by two-dimensional electrophoresis and which exhibit genetic variation in isoelectric point. Fifteen BXD recombinant inbred strains and the two parental strains were typed for these loci and also tested for ethanol acceptance. Strains exhibiting the basic allele showed significantly higher ethanol acceptance. When 19 distantly related inbred mouse strains were tested for ethanol acceptance and typed for LTW-4, it was again found that strains exhibiting the basic allele showed significantly higher ethanol acceptance.

KW - Alcohol

KW - Ethanol acceptance

KW - Inbred strain

KW - LTW-4

KW - Mouse

KW - Pharmacogenetics

KW - Protein polymorphism

KW - Recombinant inbred strain

KW - Two-dimensional electrophoresis

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Mapping of a putative genetic locus determining ethanol intake in the mouse

Abstract

Keywords

ASJC Scopus subject areas

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