Abstract
Maternal obesity adversely impacts the in utero metabolic environment, but its effect on fetal hematopoiesis remains incompletely understood. During late development, the fetal bone marrow (FBM) becomes the major site where macrophages and B lymphocytes are produced via differentiation of hematopoietic stem and progenitor cells (HSPCs). Here, we analyzed the transcriptional landscape of FBM HSPCs at single-cell resolution in fetal macaques exposed to a maternal high-fat Western-style diet (WSD) or a low-fat control diet. We demonstrate that maternal WSD induces a proinflammatory response in FBM HSPCs and fetal macrophages. In addition, maternal WSD consumption suppresses the expression of B cell development genes and decreases the frequency of FBM B cells. Finally, maternal WSD leads to poor engraftment of fetal HSPCs in nonlethally irradiated immunodeficient NOD/SCID/IL2rγ−/− mice. Collectively, these data demonstrate for the first time that maternal WSD impairs fetal HSPC differentiation and function in a translationally relevant nonhuman primate model.
Original language | English (US) |
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Pages (from-to) | 2595-2609 |
Number of pages | 15 |
Journal | Stem Cell Reports |
Volume | 17 |
Issue number | 12 |
DOIs | |
State | Published - Dec 13 2022 |
Keywords
- B lymphocytes
- bone marrow
- fetal development
- hematopoietic stem and progenitor cells
- high-fat diet
- macrophages
- maternal programming
- monocytes
- nonhuman primates
- obesity
ASJC Scopus subject areas
- Biochemistry
- Genetics
- Developmental Biology
- Cell Biology