Metabolic imaging of the organ of corti - A window on cochlea bioenergetics

Le Ann Tiede, Peter S. Steyger, Michael G. Nichols, Richard Hallworth

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


Hair cell loss is a major cause of sensorineural hearing loss. We have developed a method to examine metabolic events in hair cells in response to stimuli known to cause hair cell loss, such as acoustic trauma and aminoglycoside administration. The method employs two-photon excitation of the metabolic intermediate, reduced nicotinamide adenine dinucleotide (NADH), in hair cell mitochondria in an explanted mouse cochlea. Using this method, we show evidence that the aminoglycoside gentamicin selectively affects the level of mitochondrial NADH in outer hair cells, but not inner hair cells, within minutes of administration.

Original languageEnglish (US)
Pages (from-to)37-41
Number of pages5
JournalBrain research
StatePublished - Jun 24 2009


  • Aminoglycoside
  • Gentamicin
  • Multi-photon confocal microscopy
  • NADH
  • Sensorineural hearing loss

ASJC Scopus subject areas

  • General Neuroscience
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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