MIFlowCyt: The minimum information about a flow cytometry experiment

Jamie A. Lee, Josef Spidlen, Keith Boyce, Jennifer Cai, Nicholas Crosbie, Mark Dalphin, Jeff Furlong, Maura Gasparetto, Michael Goldberg, Elizabeth M. Goralczyk, Bill Hyun, Kirstin Jansen, Tobias Kollmann, Megan Kong, Robert Leif, Shannon McWeeney, Thomas D. Moloshok, Wayne Moore, Garry Nolan, John NolanJanko Nikolich-Zugich, David Parrish, Barclay Purcell, Yu Qian, Biruntha Selvaraj, Clayton Smith, Olga Tchuvatkina, Anne Wertheimer, Peter Wilkinson, Christopher Wilson, James Wood, Robert Zigon, Richard H. Scheuermann, Ryan R. Brinkman

Research output: Contribution to journalArticlepeer-review

350 Scopus citations


A fundamental tenet of scientific research is that published results are open to independent validation and refutation. Minimum data standards aid data providers, users, and publishers by providing a specification of what is required to unambiguously interpret experimental findings. Here, we present the Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) standard, stating the minimum information required to report flow cytometry (FCM) experiments. We brought together a cross-disciplinary international collaborative group of bioinformaticians, computational statisticians, software developers, instrument manufacturers, and clinical and basic research scientists to develop the standard. The standard was subsequently vetted by the International Society for Advancement of Cytometry (ISAC) Data Standards Task Force, Standards Committee, membership, and Council. The MIFlowCyt standard includes recommendations about descriptions of the specimens and reagents included in the FCM experiment, the configuration of the instrument used to perform the assays, and the data processing approaches used to interpret the primary output data. MIFlowCyt has been adopted as a standard by ISAC, representing the FCM scientific community including scientists as well as software and hardware manufacturers. Adoption of MIFlowCyt by the scientific and publishing communities will facilitate third-party understanding and reuse of FCM data.

Original languageEnglish (US)
Pages (from-to)926-930
Number of pages5
JournalCytometry Part A
Issue number10
StatePublished - Oct 2008


  • Fluorescence-activated cell sorting
  • Immunology
  • Knowledge representation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology


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