TY - GEN
T1 - Modeling fluorescent light distributions in scattering media
AU - Phillips, Kevin G.
AU - Jacques, Steven L.
PY - 2010
Y1 - 2010
N2 - It is hoped that the non-invasive optical characterization of physiological features of normal and diseased epithelia can be assessed through the fluorescent emission of such tissues. With a high percentage of cancers arising in the epithelium, the characterization of carcinogenesis in such tissues is imperative. Fluorescent emission from the epithelium, e.g. oral mucosa, has been shown to be sensitive to physiological features, such as cellular morphology, and the amount and types of biochemical agents present in the tissue. Efforts to distinguish the spectral signatures of diseased and healthy states of tissues from fluorescence have been confounded by the distortion of the intrinsic fluorescent signature as a result of wavelength dependent absorption and scattering within the tissue. Theoretical models of light propagation in biological media are required for understanding the distortion of the intrinsic fluorescence arising from compromised tissues. In this work we model the distortion of the intrinsic fluorescence emitted from a tissue with wavelength dependent optical properties, arising from varying blood and water content, using the radiative transport equation. As an example, we demonstrate the ability of blood and water content to distort the signal of a white light source as it is embedded deeper into a tissue.
AB - It is hoped that the non-invasive optical characterization of physiological features of normal and diseased epithelia can be assessed through the fluorescent emission of such tissues. With a high percentage of cancers arising in the epithelium, the characterization of carcinogenesis in such tissues is imperative. Fluorescent emission from the epithelium, e.g. oral mucosa, has been shown to be sensitive to physiological features, such as cellular morphology, and the amount and types of biochemical agents present in the tissue. Efforts to distinguish the spectral signatures of diseased and healthy states of tissues from fluorescence have been confounded by the distortion of the intrinsic fluorescent signature as a result of wavelength dependent absorption and scattering within the tissue. Theoretical models of light propagation in biological media are required for understanding the distortion of the intrinsic fluorescence arising from compromised tissues. In this work we model the distortion of the intrinsic fluorescence emitted from a tissue with wavelength dependent optical properties, arising from varying blood and water content, using the radiative transport equation. As an example, we demonstrate the ability of blood and water content to distort the signal of a white light source as it is embedded deeper into a tissue.
KW - Radiative transport equation
KW - Spectroscopy
KW - Tissue-light interaction
UR - http://www.scopus.com/inward/record.url?scp=77951681276&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77951681276&partnerID=8YFLogxK
U2 - 10.1117/12.842738
DO - 10.1117/12.842738
M3 - Conference contribution
AN - SCOPUS:77951681276
SN - 9780819479587
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Interactions with Tissues and Cells XXI
T2 - Optical Interactions with Tissues and Cells XXI
Y2 - 25 January 2010 through 27 January 2010
ER -