Mouse chromosome 7 harbors a quantitative trait locus for isoflurane minimum alveolar concentration

Michael Cascio, Yilei Xing, Diane Gong, John Popovich, Edmond I. Eger, Saunak Sen, Gary Peltz, James M. Sonner

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


BACKGROUND: The minimum alveolar concentration (MAC) of isoflurane is a quantitative trait because it varies continuously in a population. The location on the genome of genes or other genetic elements controlling quantiative traits is called quantitative trait loci (QTLs). In this study we sought to detect a quantitative trait locus underlying isoflurane MAC in mice. METHODS: To accomplish this, two inbred mouse strains differing in isoflurane MAC, the C57BL/6J and LP/J mouse strains, were bred through two generations to produce genetic recombination. These animals were genotyped for microsatellite markers. We also applied an independent, computational method for identifying QTL-regulating differences in isoflurane MAC. In this approach, the isoflurane MAC was measured in a panel of 19 inbred strains, and computationally searched for genomic intervals where the pattern of genetic variation, based on single nucleotide polymorphisms, correlated with the differences in isoflurane MAC among inbred strains. RESULTS AND CONCLUSIONS: Both methods of genetic analysis identified a QTL for isoflurane MAC that was located on the proximal part of mouse chromosome 7.

Original languageEnglish (US)
Pages (from-to)381-385
Number of pages5
JournalAnesthesia and analgesia
Issue number2
StatePublished - Aug 2007
Externally publishedYes

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine


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