MRNA expression of ion channels in GnRH neurons: Subtype-specific regulation by 17β-estradiol

Martha A. Bosch, Karen J. Tonsfeldt, Oline K. Rønnekleiv

Research output: Contribution to journalArticlepeer-review

72 Scopus citations


Burst firing of neurons optimizes neurotransmitter release. GnRH neurons exhibit burst firing activity and T-type calcium channels, which are vital for burst firing activity, are regulated by 17β-estradiol (E2) in GnRH neurons. To further elucidate ion channel expression and E2 regulation during positive and negative feedback on GnRH neurosecretion, we used single cell RT-PCR and real-time qPCR to quantify channel mRNA expression in GnRH neurons. GFP-GnRH neurons expressed numerous ion channels important for burst firing activity. E2-treatment sufficient to induce an LH surge increased mRNA expression of HCN1 channels, which underlie the pacemaker current, the calcium-permeable CaV1.3, CaV2.2, CaV2.3 channels, and TRPC4 channels, which mediate the kisspeptin excitatory response. E2 also decreased mRNA expression of SK3 channels underlying the medium AHP current. Therefore, E2 exerts fundamental changes in ion channel expression in GnRH neurons, to prime them to respond to incoming stimuli with increased excitability at the time of the surge.

Original languageEnglish (US)
Pages (from-to)85-97
Number of pages13
JournalMolecular and Cellular Endocrinology
Issue number1-2
StatePublished - Mar 10 2013
Externally publishedYes


  • AHP
  • Channel subunits
  • E2
  • GPR54
  • GnRH
  • GnRH neurons
  • HCN
  • HVA
  • LH
  • LH surge
  • MRNA quantification
  • POA
  • QPCR
  • RT
  • SK
  • Sc-PCR
  • TRPC
  • ZT

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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