Multiple antibiotic susceptibility associated with inactivation of the prc gene

A. Seoane, A. Sabbaj, L. M. McMurry, S. B. Levy

Research output: Contribution to journalComment/debatepeer-review

30 Scopus citations


A Tn5 insertion which led to increased susceptibility to multiple drugs, including tetracycline, chloramphenicol, nalidixic acid, erythromycin, spectinomycin, norfloxacin, and novobiocin, was identified in Escherichia coli. Cloning and sequence studies showed that the insertion was in the previously identified prc gene at min 40.4. The prc product is known to function as a protease linked to processing of penicillin-binding protein 3 and λ repressor and when absent to allow some leakage of periplasmic constituents. Complementation studies with the prc gene on plasmids showed complete recovery of parental levels of susceptibility to all drugs except chloramphenicol, with which only partial reversion to wild-type levels was observed.

Original languageEnglish (US)
Pages (from-to)7844-7847
Number of pages4
JournalJournal of bacteriology
Issue number23
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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