Myocardial contrast two-dimensional echocardiography can be an index of in vivo red blood cell transit rate

W. P. Glasheen, A. R. Jayaweera, S. Kaul

Research output: Contribution to journalConference articlepeer-review


We have previously shown the rheology of sonicated albumin microbubbles (size = 4.5 μ) to be similar to that of red blood cells (RBCs) in the microcirculation. Our hypotheses was that the myocardial transit of these microbubbles as measured by 2D-echo would be an index of myocardial RBC transit rate in vivo. We cannulated the left anterior descending (LAD) artery in 8 dogs and perfused it with blood from the right carotid artery. LAD blood flow was varied from 13 to 110 ml/min using a roller pump. At each stage, 100 μCi of 99mTechnetium labeled RBCs was injected into the LAD bed and gamma emissions were sampled over the bed every 0.5 sec to generate time-activity curves. Sonicated albumin (0.5 ml) was also injected at each stage and 2D echo was performed to obtain time-intensity curves. There was an excellent correlation between actual blood flow (ml/min) and the rate of transit of both RBC's and microbubbles (R2 = 0.98 and r2 = 0.90, respectively). There was also a close correlation between microbubble and labeled RBC transit rate during the 39 stages analyzed (y = 1.04x, SEE = 0.05, R2 = 0.92). We conclude that myocardial contrast echocardiography can be used to assess RBC transit rate in vivo. This offers a powerful tool for measuring regional myocardial blood flow.

Original languageEnglish (US)
Pages (from-to)583-584
Number of pages2
JournalAnnals of Biomedical Engineering
Issue number5
StatePublished - 1991
Externally publishedYes
Event1991 Annual Fall Meeting of the Biomedical Engineering Society - Charlottesville, VA, USA
Duration: Oct 12 1991Oct 14 1991

ASJC Scopus subject areas

  • Biomedical Engineering


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