N-methyl-d-aspartate (NMDA) receptors are inactivated by trypsin

Charles N. Allen, Robert Brady, John Swann, Nobuaki Hori, David O. Carpenter

Research output: Contribution to journalArticlepeer-review

51 Scopus citations


Acute isolation of hippocampal CA3 pyramidal cells using trypsin produces neurons which respond to kainate and quisqualate but not N-methyl-d-aspartate (NMDA). Incubation of 6- to 12-day-old cultured hippocampal neurons or slices of pyriform cortex with trypsin irreversibly removes the NMDA responses normally present without significant effect on responses to kainate or quisqualate. These data indicate that the NMDA receptor has a trypsin-sensitive component which is necessary for agonist recognition or ion channel activation.

Original languageEnglish (US)
Pages (from-to)147-150
Number of pages4
JournalBrain research
Issue number1
StatePublished - Aug 16 1988
Externally publishedYes


  • Hippocampal neuron
  • Kainate
  • N-methyl-d-aspartate
  • Pyriform cortex
  • Quisqualate
  • Trypsin

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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