TY - JOUR
T1 - Nitric oxide distribution and production in the guinea pig cochlea
AU - Shi, Xiaorui
AU - Ren, Tianying
AU - Nuttall, Alfred L.
N1 - Funding Information:
This work was supported by NIH Research Grants NIDCD R01 DC 00105 and DC 00105 25S1. The authors thank Dr. Peter Steyger for valuable help with fluorescence and cell isolation techniques, Dr. Dennis Trune and Ms. Jacqueline DeGagne for photomicrograph production.
PY - 2001
Y1 - 2001
N2 - Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.
AB - Production sites and distribution of nitric oxide (NO) were detected in cochlear lateral wall tissue, the organ of Corti and in isolated outer hair cells (OHCs) from the guinea pig using the fluorescent dye, 4,5-diaminofluorescein diacetate. Fluorescent signal, indicating the presence of NO, was found in the afferent nerves and their putative endings near inner hair cells (IHCs) and putative efferent nerve endings near OHCs, the IHCs and OHCs, the endothelial cells of blood vessels of the spiral ligament, the stria vascularis, and the spiral blood vessels of the basilar membrane. An increased NO signal was observed following exposure to the substrate for NO, L-arginine, while exposure to NO synthase inhibitors resulted in a decrease in NO signal. Observation of OHCs at the subcellular level revealed differentially strong fluorescent signals at the locations of cuticular plate, the subcuticular plate region, the infranuclear region, and the region adjacent to the lateral wall. The findings indicate the presence of NO in the cochlea and suggest that NO may play an important role in both regulating vascular tone and mediating neurotransmission in guinea pig cochlea.
KW - 4,5-Diaminofluorescein diacetate
KW - Endothelial cell
KW - L-Arginine
KW - N-Monomethyl-L-arginine monoacetate
KW - N-Nitro-L-arginine methyl ester HCl
KW - Nitric oxide
KW - Outer hair cell
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U2 - 10.1016/S0378-5955(00)00254-9
DO - 10.1016/S0378-5955(00)00254-9
M3 - Article
C2 - 11223294
AN - SCOPUS:0035133829
SN - 0378-5955
VL - 153
SP - 23
EP - 31
JO - Hearing Research
JF - Hearing Research
IS - 1-2
ER -