Nitric oxide facilitates N-methyl-D-aspartate-induced burst firing in dopamine neurons from rat midbrain slices

Barbara A. Cox; Steven W. Johnson

doi:10.1016/S0304-3940(98)00737-X

Nitric oxide facilitates N-methyl-D-aspartate-induced burst firing in dopamine neurons from rat midbrain slices

Barbara A. Cox, Steven W. Johnson

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Dopamine (DA) neurons in the ventral tegmental area and substantia nigra pars compacta were induced to fire in bursts with application of N-methyl-D- aspartate (NMDA, 20 μM) and apamin (100 nM) while recording intracellularly in the rat brain slice. L-Arginine (300 μM), a substrate for nitric oxide (NO) production, increased both the number of spikes per burst and the magnitude of interburst hyperpolarizations. Nitric oxide synthase inhibitors N-nitro-L-arginine methyl ester (L-NAME, 100 μM), N-nitro-L-arginine, and 7- nitroindazole inhibited NMDA-induced burst firing by reducing the number of spikes per burst. Moreover, L-arginine (100 μM) reversed the inhibition of burst firing produced by L-NAME. These findings suggest that NO facilitates NMDA-induced burst firing in DA neurons.

Original language	English (US)
Pages (from-to)	131-134
Number of pages	4
Journal	Neuroscience Letters
Volume	255
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(98)00737-X
State	Published - Oct 23 1998
Externally published	Yes

Keywords

Brain slice
Burst firing
Dopamine neuron
L-Arginine
N- nitro-L-arginine methyl ester
N-Methyl-D-aspartate
Nitric oxide
Substantia nigra
Ventral tegmental area

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(98)00737-X

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title = "Nitric oxide facilitates N-methyl-D-aspartate-induced burst firing in dopamine neurons from rat midbrain slices",

abstract = "Dopamine (DA) neurons in the ventral tegmental area and substantia nigra pars compacta were induced to fire in bursts with application of N-methyl-D- aspartate (NMDA, 20 μM) and apamin (100 nM) while recording intracellularly in the rat brain slice. L-Arginine (300 μM), a substrate for nitric oxide (NO) production, increased both the number of spikes per burst and the magnitude of interburst hyperpolarizations. Nitric oxide synthase inhibitors N-nitro-L-arginine methyl ester (L-NAME, 100 μM), N-nitro-L-arginine, and 7- nitroindazole inhibited NMDA-induced burst firing by reducing the number of spikes per burst. Moreover, L-arginine (100 μM) reversed the inhibition of burst firing produced by L-NAME. These findings suggest that NO facilitates NMDA-induced burst firing in DA neurons.",

keywords = "Brain slice, Burst firing, Dopamine neuron, L-Arginine, N- nitro-L-arginine methyl ester, N-Methyl-D-aspartate, Nitric oxide, Substantia nigra, Ventral tegmental area",

author = "Cox, {Barbara A.} and Johnson, {Steven W.}",

note = "Funding Information: This work was supported by grants from the National Institute of Mental Health (MH40416), The Scottish Rite Benevolent Foundation's Schizophrenia Research Program (S.W.J. and B.A.C.), and the National Alliance for Research on Schizophrenia and Depression (B.A.C).",

year = "1998",

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doi = "10.1016/S0304-3940(98)00737-X",

language = "English (US)",

volume = "255",

pages = "131--134",

journal = "Neuroscience Letters",

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T1 - Nitric oxide facilitates N-methyl-D-aspartate-induced burst firing in dopamine neurons from rat midbrain slices

AU - Cox, Barbara A.

AU - Johnson, Steven W.

N1 - Funding Information: This work was supported by grants from the National Institute of Mental Health (MH40416), The Scottish Rite Benevolent Foundation's Schizophrenia Research Program (S.W.J. and B.A.C.), and the National Alliance for Research on Schizophrenia and Depression (B.A.C).

PY - 1998/10/23

Y1 - 1998/10/23

N2 - Dopamine (DA) neurons in the ventral tegmental area and substantia nigra pars compacta were induced to fire in bursts with application of N-methyl-D- aspartate (NMDA, 20 μM) and apamin (100 nM) while recording intracellularly in the rat brain slice. L-Arginine (300 μM), a substrate for nitric oxide (NO) production, increased both the number of spikes per burst and the magnitude of interburst hyperpolarizations. Nitric oxide synthase inhibitors N-nitro-L-arginine methyl ester (L-NAME, 100 μM), N-nitro-L-arginine, and 7- nitroindazole inhibited NMDA-induced burst firing by reducing the number of spikes per burst. Moreover, L-arginine (100 μM) reversed the inhibition of burst firing produced by L-NAME. These findings suggest that NO facilitates NMDA-induced burst firing in DA neurons.

AB - Dopamine (DA) neurons in the ventral tegmental area and substantia nigra pars compacta were induced to fire in bursts with application of N-methyl-D- aspartate (NMDA, 20 μM) and apamin (100 nM) while recording intracellularly in the rat brain slice. L-Arginine (300 μM), a substrate for nitric oxide (NO) production, increased both the number of spikes per burst and the magnitude of interburst hyperpolarizations. Nitric oxide synthase inhibitors N-nitro-L-arginine methyl ester (L-NAME, 100 μM), N-nitro-L-arginine, and 7- nitroindazole inhibited NMDA-induced burst firing by reducing the number of spikes per burst. Moreover, L-arginine (100 μM) reversed the inhibition of burst firing produced by L-NAME. These findings suggest that NO facilitates NMDA-induced burst firing in DA neurons.

KW - Brain slice

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KW - Dopamine neuron

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KW - N- nitro-L-arginine methyl ester

KW - N-Methyl-D-aspartate

KW - Nitric oxide

KW - Substantia nigra

KW - Ventral tegmental area

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Nitric oxide facilitates N-methyl-D-aspartate-induced burst firing in dopamine neurons from rat midbrain slices

Abstract

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