Nutritional Regulation of Insulin-like Growth Factor-I Receptor mRNA Levels in Growing Chickens

Yuki Matsumura, Manabu Domeki, Kunio Sugahara, Tatsuo Kubo, Charles T. Roberts, Derek Le Roith, Hisanori Kato

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Insulin-like growth factor-I (IGF-I) exerts its effect through the IGF-I receptor. To investigate the effects of nutritional status on chicken IGF-I receptor gene expression, a solution hybridization/RNase protection assay for IGF-I receptor mRNA was developed. A cDNA clone corresponding to the carboxyl- terminal region of the IGF-I receptor was obtained by reverse transcription-PCR (RT-PCR). Sequence analysis of the clone showed that this region of the chicken IGF-I receptor is highly divergent from the human IGF-I receptor. IGF-I receptor mRNA was detected in all tissues examined from newly hatched chickens. The rank order of the IGF-I receptor mRNA levels was liver < thigh muscle < stomach < heart < lung < kidney < brain. In 1-week-old chickens, 5 days of starvation caused a 2.5- to 3-fold increase in the mRNA in muscle and kidney. Starvation of 4-week-old chickens for 5 days caused a 1.7 to 2.2-fold increase in IGF-I receptor mRNA levels in kidney, liver, and muscle. In contrast, IGF-I receptor mRNA levels in brain failed to change. The mRNA levels were reduced to the control level by refeeding of the starved chickens for 24 h. These data suggest a tissue- and development-specific response of chicken IGF-I receptor gene expression to nutritional status.

Original languageEnglish (US)
Pages (from-to)979-982
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Issue number6
StatePublished - Jan 1996


  • Chicken
  • Growth factor receptor
  • Insulin-like growth factor-I
  • R Nase protection assay
  • Starvation

ASJC Scopus subject areas

  • General Medicine


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