Optical coherence tomography angiography of peripapillary retinal blood flow response to hyperoxia

Alex D. Pechauer, Yali Jia, Liang Liu, Simon S. Gao, Chunhui Jiang, David Huang

Research output: Contribution to journalArticlepeer-review

102 Scopus citations


Purpose. To measure the change in peripapillary retinal blood flow in response to hyperoxia by using optical coherence tomography (OCT) angiography. Methods. One eye of each healthy human participants (six) was scanned with a commercial high-speed (70 kHz) spectral OCT. Scans were captured twice after 10-minute exposures to normal breathing (baseline) and hyperoxia. Blood flow was detected by the split-spectrum amplitude-decorrelation angiography (SSADA) algorithm. Peripapillary retinal blood flow index and vessel density were calculated from en face maximum projections of the retinal layers. The experiment was performed on 2 separate days for each participant. Coefficient of variation (CV) was used to measure within-day repeatability and between-day reproducibility. Paired t-tests were used to compare means of baseline and hyperoxic peripapillary retinal blood low. Results. A decrease of 8.87% ± 3.09% (mean ± standard deviation) in flow index and 2.61% ± 1.50% in vessel density was observed under hyperoxia. The within-day repeatability CV of baseline measurements was 5.75% for flow index and 1.67% for vessel density. The between-day reproducibility CV for baseline flow index and vessel density was 11.1% and 1.14%, respectively. The between-day reproducibility of the hyperoxic response was 3.71% and 1.67% for low index and vessel density, respectively. Conclusions. Optical coherence tomography angiography with SSADA was able to detect a decrease in peripapillary retinal blood flow in response to hyperoxia. The response was larger than the variability of baseline measurements. The magnitude of an individual's hyperoxic response was highly variable between days. Thus, reliable assessment may require averaging multiple measurements.

Original languageEnglish (US)
Pages (from-to)3287-3291
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Issue number5
StatePublished - 2015


  • Angiography
  • Hyperoxia
  • Optical coherence tomography
  • Peripapillary retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


Dive into the research topics of 'Optical coherence tomography angiography of peripapillary retinal blood flow response to hyperoxia'. Together they form a unique fingerprint.

Cite this