TY - JOUR
T1 - Optimising the yield from bronchoalveolar lavage on human participants in infectious disease immunology research
AU - The Stellenbosch University Immunology Research Group Bronchoalveolar Lavage Study Consortium (SU IRG BAL Study Consortium)
AU - Shaw, Jane Alexandra
AU - Meiring, Maynard
AU - Allies, Devon
AU - Cruywagen, Lauren
AU - Fisher, Tarryn Lee
AU - Kasavan, Kesheera
AU - Roos, Kelly
AU - Botha, Stefan Marc
AU - MacDonald, Candice
AU - Hiemstra, Andriёtte M.
AU - Simon, Donald
AU - van Rensburg, Ilana
AU - Flinn, Marika
AU - Shabangu, Ayanda
AU - Kuivaniemi, Helena
AU - Tromp, Gerard
AU - Malherbe, Stephanus T.
AU - Walzl, Gerhard
AU - du Plessis, Nelita
AU - Nemes, Elisa
AU - Kleynhans, Léanie
AU - McAnda, Shirley
AU - Kruger, Charlene
AU - Richardson, Tracey
AU - Noor, Firdows
AU - Benting, Lauren
AU - Mtala, Nosipho
AU - Storm, Robin Lee
AU - Persence, Gertrude
AU - Molima, Elsabe
AU - Chetram, Alicia
AU - Stanley, Kim
AU - Lewinsohn, David M.
AU - Lewinsohn, Deborah A.
AU - Urdahl, Kevin B.
AU - Schurr, Erwin
AU - Orlova, Marianna
AU - Boom, W. Henry
AU - Fortune, Sarah
N1 - Publisher Copyright:
© 2023, The Author(s).
PY - 2023/12
Y1 - 2023/12
N2 - Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, but this declined at the end of treatment. HIV infection was associated with more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells.
AB - Bronchoalveolar lavage (BAL) is becoming a common procedure for research into infectious disease immunology. Little is known about the clinical factors which influence the main outcomes of the procedure. In research participants who underwent BAL according to guidelines, the BAL volume yield, and cell yield, concentration, viability, pellet colour and differential count were analysed for association with important participant characteristics such as active tuberculosis (TB) disease, TB exposure, HIV infection and recent SARS-CoV-2 infection. In 337 participants, BAL volume and BAL cell count were correlated in those with active TB disease, and current smokers. The right middle lobe yielded the highest volume. BAL cell and volume yields were lower in older participants, who also had more neutrophils. Current smokers yielded lower volumes and higher numbers of all cell types, and usually had a black pellet. Active TB disease was associated with higher cell yields, but this declined at the end of treatment. HIV infection was associated with more bloody pellets, and recent SARS-CoV-2 infection with a higher proportion of lymphocytes. These results allow researchers to optimise their participant and end assay selection for projects involving lung immune cells.
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U2 - 10.1038/s41598-023-35723-2
DO - 10.1038/s41598-023-35723-2
M3 - Article
C2 - 37258565
AN - SCOPUS:85160755201
SN - 2045-2322
VL - 13
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 8859
ER -