Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus

Denise I. Quigley; John McDougall; Tristan Darland; Ge Zhang; Oline Ronnekliev; David K. Grandy; Richard G. Allen

doi:10.1016/S0196-9781(97)00268-4

Orphanin FQ is the major OFQ^1-17-containing peptide produced in the rodent and monkey hypothalamus

Denise I. Quigley, John McDougall, Tristan Darland, Ge Zhang, Oline Ronnekliev, David K. Grandy, Richard G. Allen

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed- phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co- eluting with synthetic OFQ^1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ^1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.

Original language	English (US)
Pages (from-to)	133-139
Number of pages	7
Journal	Peptides
Volume	19
Issue number	1
DOIs	https://doi.org/10.1016/S0196-9781(97)00268-4
State	Published - 1998
Externally published	Yes

Keywords

Hypothalamus
Neuropeptides
Orphanin FQ
Processing

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Access to Document

10.1016/S0196-9781(97)00268-4

Cite this

@article{ae02e69feb4b4e71bf8a4a800a2c6acd,

title = "Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus",

abstract = "In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed- phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co- eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.",

keywords = "Hypothalamus, Neuropeptides, Orphanin FQ, Processing",

author = "Quigley, {Denise I.} and John McDougall and Tristan Darland and Ge Zhang and Oline Ronnekliev and Grandy, {David K.} and Allen, {Richard G.}",

note = "Funding Information: We would like to thank Dan Austin for the graphics. We would also like to thank Dr. Malcolm Low for critical reading of the manuscript. We thank Emily McKinzie for excellent secretarial assistance. This work was supported by NIH grants HD30236 (RGA) and DA08562 (DKG), a grant from the Markey Charitable Trust (DKG) and a grant from the Medical Research Foundation of Oregon (RGA).",

year = "1998",

doi = "10.1016/S0196-9781(97)00268-4",

language = "English (US)",

volume = "19",

pages = "133--139",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "1",

}

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T1 - Orphanin FQ is the major OFQ1-17-containing peptide produced in the rodent and monkey hypothalamus

AU - Quigley, Denise I.

AU - McDougall, John

AU - Darland, Tristan

AU - Zhang, Ge

AU - Ronnekliev, Oline

AU - Grandy, David K.

AU - Allen, Richard G.

N1 - Funding Information: We would like to thank Dan Austin for the graphics. We would also like to thank Dr. Malcolm Low for critical reading of the manuscript. We thank Emily McKinzie for excellent secretarial assistance. This work was supported by NIH grants HD30236 (RGA) and DA08562 (DKG), a grant from the Markey Charitable Trust (DKG) and a grant from the Medical Research Foundation of Oregon (RGA).

PY - 1998

Y1 - 1998

N2 - In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed- phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co- eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.

AB - In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed- phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co- eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.

KW - Hypothalamus

KW - Neuropeptides

KW - Orphanin FQ

KW - Processing

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