Osmotic response in Lactobacillus casei ATCC 393: Biochemical and biophysical characteristics of membrane

M. Cecilia Machado; Claudia S. López; Horacio Heras; Emilio A. Rivas

doi:10.1016/j.abb.2003.11.001

Osmotic response in Lactobacillus casei ATCC 393: Biochemical and biophysical characteristics of membrane

M. Cecilia Machado, Claudia S. López, Horacio Heras, Emilio A. Rivas

Pacific Northwest Center for Cryo-EM (PNCC)

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

The biochemical and biophysical properties of the membrane and some general characteristics of the response of Lactobacillus casei ATCC 393 (reclassified Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.

Original language	English (US)
Pages (from-to)	61-70
Number of pages	10
Journal	Archives of Biochemistry and Biophysics
Volume	422
Issue number	1
DOIs	https://doi.org/10.1016/j.abb.2003.11.001
State	Published - Feb 1 2004

Keywords

Bile salts
Fluorescent probes
Lactobacillus casei
Lipids
Membranes
Osmotic stress
Permeability

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/j.abb.2003.11.001

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title = "Osmotic response in Lactobacillus casei ATCC 393: Biochemical and biophysical characteristics of membrane",

abstract = "The biochemical and biophysical properties of the membrane and some general characteristics of the response of Lactobacillus casei ATCC 393 (reclassified Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.",

keywords = "Bile salts, Fluorescent probes, Lactobacillus casei, Lipids, Membranes, Osmotic stress, Permeability",

author = "Machado, {M. Cecilia} and L{\'o}pez, {Claudia S.} and Horacio Heras and Rivas, {Emilio A.}",

note = "Funding Information: This work was supported by grants from CONICET, Argentina. C.S.L was a doctoral fellow and H.H. and E.A.R are members of the Carrera del Investigador Cient{\'ı}fico, CONICET, Argentina. We are grateful to Dr. H. Viola for statistical calculations and to Drs. S. Ruzal and C. S{\'a}nchez-Rivas for the critical reading of the manuscript.",

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AU - Heras, Horacio

AU - Rivas, Emilio A.

N1 - Funding Information: This work was supported by grants from CONICET, Argentina. C.S.L was a doctoral fellow and H.H. and E.A.R are members of the Carrera del Investigador Cientı́fico, CONICET, Argentina. We are grateful to Dr. H. Viola for statistical calculations and to Drs. S. Ruzal and C. Sánchez-Rivas for the critical reading of the manuscript.

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N2 - The biochemical and biophysical properties of the membrane and some general characteristics of the response of Lactobacillus casei ATCC 393 (reclassified Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.

AB - The biochemical and biophysical properties of the membrane and some general characteristics of the response of Lactobacillus casei ATCC 393 (reclassified Lactobacillus zeae) to hyperosmotic conditions were studied. Under hypertonic conditions, the hydrophobicity and the bile salt sensitivity of the cultures were increased. The glycolipid AcylH3DG is only present in membranes of NaCl containing medium, whereas, H4DG undergoes a significant increment and H2DG a significant decrease. The fluidity of both the purified membranes and the total lipid vesicles, as determined with the fluorescent probe DPH, did not change in conditions of high salinity. This was coincident with changes in the fatty acid (FA) composition where an increase in the saturated/unsaturated FA ratio was compensated by a rise in the fluidifying 11,12-methyleneoctadecanoic FA (cyc 19:0). Under osmotic stress conditions, Laurdan and acridine orange in total lipid vesicles showed increased lateral lipid packing and proton permeability, respectively.

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KW - Osmotic stress

KW - Permeability

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Osmotic response in Lactobacillus casei ATCC 393: Biochemical and biophysical characteristics of membrane

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Keywords

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