TY - JOUR
T1 - Papain gel containing methylene blue for simultaneous caries removal and antimicrobial photoinactivation against Streptococcus mutans biofilms
AU - Silva, Zenildo Santos
AU - Huang, Ying Ying
AU - De Freitas, Lucas Freitas
AU - França, Cristiane Miranda
AU - Botta, Sergio Brossi
AU - Ana, Patrícia Aparecida
AU - Mesquita-Ferrari, Raquel Agnelli
AU - Santos Fernandes, Kristianne Porta
AU - Deana, Alessandro
AU - Lima Leal, Cintia Raquel
AU - Prates, Renato Araujo
AU - Hamblin, Michael R.
AU - Bussadori, Sandra Kalil
PY - 2016/9/19
Y1 - 2016/9/19
N2 - This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue-MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films. Cytotoxicity and subcellular localization was studied in human fibroblasts. Sponges of bioabsorbable type I collagen membrane were exposed to papain based gel, irradiated with laser and analyzed about their integrity by ATR-FTIR. The PapaMBlue produced higher amounts of singlet oxygen and hydroxyl radicals than free MB, possibly due to better disaggregation of the dye in solution. The PapaMBlue antimicrobial effects on biofilms proved to be capable of reducing the S. mutans. Both MTT and PrestoBlue assays showed higher cell viability and metabolism scores in fibroblasts treated with PapaMBlue and MB, possibly due to stimulation of mitochondrial activity and that collagen triple helix is unaffected. The PapaMBlue is equally effective as MB in destroying S. mutans biofilms growing on plastic or collagen without affecting fibroblasts.
AB - This study intended to evaluate the effects of a papain-gel with a red-light absorbing pigment (methylene blue-MB) to mediate photodynamic therapy (PDT) against Streptococcus mutans biofilms. The PapaMBlue was compared with free MB to generate reactive oxygen species using fluorescence probes (SOSG and HPF). PDT (660-nm light) was carried out against S. mutans biofilms grown on either plastic dishes or on collagen membrane and assayed by CFU, live-dead staining using confocal microscopy, transmission electron microscopy and H&E staining for collagen films. Cytotoxicity and subcellular localization was studied in human fibroblasts. Sponges of bioabsorbable type I collagen membrane were exposed to papain based gel, irradiated with laser and analyzed about their integrity by ATR-FTIR. The PapaMBlue produced higher amounts of singlet oxygen and hydroxyl radicals than free MB, possibly due to better disaggregation of the dye in solution. The PapaMBlue antimicrobial effects on biofilms proved to be capable of reducing the S. mutans. Both MTT and PrestoBlue assays showed higher cell viability and metabolism scores in fibroblasts treated with PapaMBlue and MB, possibly due to stimulation of mitochondrial activity and that collagen triple helix is unaffected. The PapaMBlue is equally effective as MB in destroying S. mutans biofilms growing on plastic or collagen without affecting fibroblasts.
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U2 - 10.1038/srep33270
DO - 10.1038/srep33270
M3 - Article
C2 - 27641507
AN - SCOPUS:84988451688
SN - 2045-2322
VL - 6
JO - Scientific Reports
JF - Scientific Reports
M1 - 33270
ER -