PASTA: PARP activity screening and inhibitor testing assay

Ilsa T. Kirby; Daniel J. Sanderson; Michael S. Cohen

doi:10.1016/j.xpro.2021.100344

PASTA: PARP activity screening and inhibitor testing assay

Ilsa T. Kirby, Daniel J. Sanderson, Michael S. Cohen

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).

Original language	English (US)
Article number	100344
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.100344
State	Published - Mar 19 2021

Keywords

High-throughput screening
Molecular/chemical probes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Neuroscience(all)
Immunology and Microbiology(all)
Medicine(all)

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10.1016/j.xpro.2021.100344

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title = "PASTA: PARP activity screening and inhibitor testing assay",

abstract = "Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).",

keywords = "High-throughput screening, Molecular/chemical probes",

author = "Kirby, {Ilsa T.} and Sanderson, {Daniel J.} and Cohen, {Michael S.}",

note = "Funding Information: We thank Dr. Ian Carter-O{\textquoteright}Connell and Dr. Rory Morgan for helpful discussions and advice to I.T.K. while developing this assay. This work was funded by the NIH (NIH 2R01NS088629 ) and the Pew Biomedical Foundation to M.S.C. Publisher Copyright: {\textcopyright} 2021 The Authors",

year = "2021",

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doi = "10.1016/j.xpro.2021.100344",

language = "English (US)",

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T2 - PARP activity screening and inhibitor testing assay

AU - Kirby, Ilsa T.

AU - Sanderson, Daniel J.

AU - Cohen, Michael S.

N1 - Funding Information: We thank Dr. Ian Carter-O’Connell and Dr. Rory Morgan for helpful discussions and advice to I.T.K. while developing this assay. This work was funded by the NIH (NIH 2R01NS088629 ) and the Pew Biomedical Foundation to M.S.C. Publisher Copyright: © 2021 The Authors

PY - 2021/3/19

Y1 - 2021/3/19

N2 - Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).

AB - Small-molecule inhibitors have been instrumental in uncovering the biological importance of poly-ADP-ribose polymerases (PARPs), a family of enzymes involved in wide-ranging aspects of cell biology. However, few PARP inhibitors are tested against the entire family of PARPs. This makes it impossible to confidently assess the role of a single PARP in cellular processes using small molecules. Here, we detail a PARP activity screening and inhibitor testing assay (PASTA) for determining relative selectivity of PARP inhibitors. For complete details on the use and execution of this protocol, please refer to Kirby et al. (2018).

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PASTA: PARP activity screening and inhibitor testing assay

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Keywords

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