PH-Induced Binding of the Axial Ligand in an Engineered CuA Site Favors the πu State

Marcos N. Morgada, Florencia Emiliani, Kelly N. Chacón, Damián Álvarez-Paggi, Daniel H. Murgida, Ninian J. Blackburn, Luciano A. Abriata, Alejandro J. Vila

Research output: Contribution to journalArticlepeer-review


CuA centers perform efficient long-range electron transfer. The electronic structure of native CuA sites can be described by a double-potential well with a dominant σu∗ ground state in fast equilibrium with a less populated πu ground state. Here, we report a CuA mutant in which a lysine was introduced in the axial position. This results in a highly unstable protein with a pH-dependent population of the two ground states. Deep analysis of the high-pH form of this variant shows the stabilization of the πu ground state due to direct binding of the Lys residue to the copper center that we attribute to deprotonation of this residue.

Original languageEnglish (US)
Pages (from-to)15687-15691
Number of pages5
JournalInorganic Chemistry
Issue number23
StatePublished - Dec 2 2019

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Inorganic Chemistry


Dive into the research topics of 'PH-Induced Binding of the Axial Ligand in an Engineered CuA Site Favors the πu State'. Together they form a unique fingerprint.

Cite this