Phosphoregulatory protein 14-3-3 facilitates SAC1 transport from the endoplasmic reticulum

Kanika Bajaj Pahuja, Jinzhi Wang, Anastasia Blagoveshchenskaya, Lillian Lim, M. S. Madhusudhan, Peter Mayinger, Randy Schekman

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Most secretory cargo proteins in eukaryotes are synthesized in the endoplasmic reticulum and actively exported in membrane-bound vesicles that are formed by the cytosolic coat protein complex II (COPII). COPII proteins are assisted by a variety of cargo-specific adaptor proteins required for the concentration and export of secretory proteins from the endoplasmic reticulum (ER). Adaptor proteins are key regulators of cargo export, and defects in their function may result in disease phenotypes in mammals. Here we report the role of 14-3-3 proteins as a cytosolic adaptor in mediating SAC1 transport in COPII-coated vesicles. Sac1 is a phosphatidyl inositol-4 phosphate (PI4P) lipid phosphatase that undergoes serum dependent translocation between the endoplasmic reticulum and Golgi complex and controls cellular PI4P lipid levels. We developed a cell-free COPII vesicle budding reaction to examine SAC1 exit from the ER that requires COPII and at least one additional cytosolic factor, the 14-3-3 protein. Recombinant 14-3-3 protein stimulates the packaging of SAC1 into COPII vesicles and the sorting subunit of COPII, Sec24, interacts with 14-3-3. We identified a minimal sorting motif of SAC1 that is important for 14-3-3 binding and which controls SAC1 export from the ER. This LS motif is part of a 7-aa stretch, RLSNTSP, which is similar to the consensus 14-3-3 binding sequence. Homology models, based on the SAC1 structure from yeast, predict this region to be in the exposed exterior of the protein. Our data suggest a model in which the 14-3-3 protein mediates SAC1 traffic from the ER through direct interaction with a sorting signal and COPII.

Original languageEnglish (US)
Pages (from-to)E3199-E3206
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number25
StatePublished - Jun 23 2015


  • 14-3-3
  • Adaptor protein
  • COPII vesicles
  • Reconstitution
  • SAC1

ASJC Scopus subject areas

  • General


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