Preparation of multivesicular liposomes

Sinil Kim, Mitchell S. Turker, Emil Y. Chi, Shifra Sela, George M. Martin

Research output: Contribution to journalArticlepeer-review

173 Scopus citations


A novel type of liposome, named here multivesicular liposomes, was prepared by evaporation of organic solvents from chloroform-ether spherules suspended in water. Within each spherule were numerous water droplets that contained solutes to be trapped in liposomes upon solvent evaporation. Liposome preparations of different average diameters were made, varying from 29 ± 10 μm to 5.6 ± 1.7 μm. The liposomes were morphologically characterized by light microscopy and transmission electron microscopy. Materials successfully trapped within the liposomes ranged in molecular size from glucose to nucleic acids. Extremely high percentages of encapsulation (up to 89%) were achieved.

Original languageEnglish (US)
Pages (from-to)339-348
Number of pages10
JournalBBA - Biomembranes
Issue number3
StatePublished - Mar 9 1983
Externally publishedYes


  • Encapsulation
  • Liposome preparation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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