TY - JOUR
T1 - Primary and secondary motoneurons use different calcium channel types to control escape and swimming behaviors in zebrafish
AU - Wen, Hua
AU - Eckenstein, Kazumi
AU - Weihrauch, Vivien
AU - Stigloher, Christian
AU - Brehm, Paul
N1 - Funding Information:
We thank Dr. Claudia Lopez from the Oregon Health and Science University Multiscale Microscope Core for her help with electron microscopy, and James Kelly for fish care. We are grateful for support with sample preparation toMarlene Strobel, Daniela Bunsen, and Claudia Gehrig-Höhn at the Imaging Core Facility of the Biocenter, University of Würzburg. This work was supported by a grant to P.B. from the NIH.
Funding Information:
ACKNOWLEDGMENTS. We thank Dr. Claudia Lopez from the Oregon Health and Science University Multiscale Microscope Core for her help with electron microscopy, and James Kelly for fish care. We are grateful for support with sample preparation to Marlene Strobel, Daniela Bunsen, and Claudia Gehrig-Höhn at the Imaging Core Facility of the Biocenter, University of Würzburg. This work was supported by a grant to P.B. from the NIH.
Publisher Copyright:
© 2020 National Academy of Sciences. All rights reserved.
PY - 2020/10/20
Y1 - 2020/10/20
N2 - The escape response and rhythmic swimming in zebrafish are distinct behaviors mediated by two functionally distinct motoneuron (Mn) types. The primary (1°Mn) type depresses and has a large quantal content (Qc) and a high release probability (Pr). Conversely, the secondary (2°Mn) type facilitates and has low and variable Qc and Pr. This functional duality matches well the distinct associated behaviors, with the 1°Mn providing the strong, singular C bend initiating escape and the 2°Mn conferring weaker, rhythmic contractions. Contributing to these functional distinctions is our identification of P/Q-type calcium channels mediating transmitter release in 1°Mns and N-type channels in 2°Mns. Remarkably, despite these functional and behavioral distinctions, all ~15 individual synapses on each muscle cell are shared by a 1°Mn bouton and at least one 2°Mn bouton. This blueprint of synaptic sharing provides an efficient way of controlling two different behaviors at the level of a single postsynaptic cell.
AB - The escape response and rhythmic swimming in zebrafish are distinct behaviors mediated by two functionally distinct motoneuron (Mn) types. The primary (1°Mn) type depresses and has a large quantal content (Qc) and a high release probability (Pr). Conversely, the secondary (2°Mn) type facilitates and has low and variable Qc and Pr. This functional duality matches well the distinct associated behaviors, with the 1°Mn providing the strong, singular C bend initiating escape and the 2°Mn conferring weaker, rhythmic contractions. Contributing to these functional distinctions is our identification of P/Q-type calcium channels mediating transmitter release in 1°Mns and N-type channels in 2°Mns. Remarkably, despite these functional and behavioral distinctions, all ~15 individual synapses on each muscle cell are shared by a 1°Mn bouton and at least one 2°Mn bouton. This blueprint of synaptic sharing provides an efficient way of controlling two different behaviors at the level of a single postsynaptic cell.
KW - Conotoxins
KW - N-type calcium channels
KW - Neuromuscular
KW - P/Q-type calcium channels
KW - Synaptic
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U2 - 10.1073/pnas.2015866117
DO - 10.1073/pnas.2015866117
M3 - Article
C2 - 33020266
AN - SCOPUS:85093829047
SN - 0027-8424
VL - 117
SP - 26429
EP - 26437
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 42
ER -