TY - JOUR
T1 - Production of extracellular matrix proteins by human pulp fibroblasts in contact with papacárie and carisolv
AU - Bussadori, Sandra Kalil
AU - Amancio, Olga Maria Silverio
AU - Martins, Manoela Domingues
AU - Guedes, Carolina Cardoso
AU - Alfaya, Thays Almeida
AU - Santos, Elaine Marcílio
AU - França, Cristiane Miranda
N1 - Publisher Copyright:
© Quintessenz all rights reserved.
PY - 2014
Y1 - 2014
N2 - Purpose: To evaluate the effect of two products for the chemomecanical removal of carious tissue (Papacárie and Carisolv) on human dental pulp fibroblasts and the synthesis of extracellular matrix proteins. Materials and Methods: Fibroblasts were divided into three groups: group 1 (control), group 2 (Papacárie) and group 3 (Carisolv). Collagen I, III, fibronectin and osteonectin were analysed by immunofluorescence and compared among the groups. Results: The groups exhibited similar immunolabeling for vimentin, type I collagen and fibronectin, but were negative for type III collagen. Osteonectin staining was strongly positive in the cells treated with Papacárie and Carisolv and weakly positive in the control group. Conclusion: The findings of the present study showed that Papacárie and Carisolv are not cytotoxic to pulp fibroblast cells. Moreover, these products stimulate fibroblasts to produce osteonectin, likely leading to the formation of dentin matrix. These findings confirm the safe, beneficial use of both gels in minimally invasive techniques.
AB - Purpose: To evaluate the effect of two products for the chemomecanical removal of carious tissue (Papacárie and Carisolv) on human dental pulp fibroblasts and the synthesis of extracellular matrix proteins. Materials and Methods: Fibroblasts were divided into three groups: group 1 (control), group 2 (Papacárie) and group 3 (Carisolv). Collagen I, III, fibronectin and osteonectin were analysed by immunofluorescence and compared among the groups. Results: The groups exhibited similar immunolabeling for vimentin, type I collagen and fibronectin, but were negative for type III collagen. Osteonectin staining was strongly positive in the cells treated with Papacárie and Carisolv and weakly positive in the control group. Conclusion: The findings of the present study showed that Papacárie and Carisolv are not cytotoxic to pulp fibroblast cells. Moreover, these products stimulate fibroblasts to produce osteonectin, likely leading to the formation of dentin matrix. These findings confirm the safe, beneficial use of both gels in minimally invasive techniques.
KW - Dental pulp
KW - Extracellular matrix proteins
KW - Osteonectin
KW - Type I collagen
KW - Type III collagen
UR - http://www.scopus.com/inward/record.url?scp=85012106652&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85012106652&partnerID=8YFLogxK
U2 - 10.3290/j.ohpd.a31225
DO - 10.3290/j.ohpd.a31225
M3 - Article
AN - SCOPUS:85012106652
SN - 1602-1622
VL - 12
SP - 55
EP - 59
JO - Oral Health and Preventive Dentistry
JF - Oral Health and Preventive Dentistry
IS - 1
ER -