Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract

L. L. David; L. J. Takemoto; R. S. Anderson; T. R. Shearer

doi:10.3109/02713688809031791

Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract

L. L. David, L. J. Takemoto, R. S. Anderson, T. R. Shearer

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Experimental nuclear cataract produced by an overdose of sodium selenite exhibited limited proteolysis, including breakdown of main intrinsic polypeptide (MIP26) to 24 and 22 kD fragments (1). Micro-sequencing and site specific immunologic probes were used in the present study to determine regions of cleavage in MIP26 during selenite cataractogenesis. Data suggested that proteolysis occurred in the C-terminus of MIP26. This may have lead to exposure of normally hidden amino acid residues on the C-terminal extension of MIP26. Loss of antigenicity of the N-terminus occurred. These significant changes to the MIP26 molecule might adversely affect communication between lens fiber cells and contribute to selenite cataract.

Original language	English (US)
Pages (from-to)	411-417
Number of pages	7
Journal	Current Eye Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.3109/02713688809031791
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract",

abstract = "Experimental nuclear cataract produced by an overdose of sodium selenite exhibited limited proteolysis, including breakdown of main intrinsic polypeptide (MIP26) to 24 and 22 kD fragments (1). Micro-sequencing and site specific immunologic probes were used in the present study to determine regions of cleavage in MIP26 during selenite cataractogenesis. Data suggested that proteolysis occurred in the C-terminus of MIP26. This may have lead to exposure of normally hidden amino acid residues on the C-terminal extension of MIP26. Loss of antigenicity of the N-terminus occurred. These significant changes to the MIP26 molecule might adversely affect communication between lens fiber cells and contribute to selenite cataract.",

author = "David, {L. L.} and Takemoto, {L. J.} and Anderson, {R. S.} and Shearer, {T. R.}",

note = "Funding Information: ACKN3LEDGEMENTS This study was partially supported by NIH grants EY-03600 and EY-03856 to TRS and NIH grant EY-02932 to WT. The authors wish to thank Michael D. Varnum for expert technical assistance.",

year = "1988",

doi = "10.3109/02713688809031791",

language = "English (US)",

volume = "7",

pages = "411--417",

journal = "Current Eye Research",

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TY - JOUR

T1 - Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract

AU - David, L. L.

AU - Takemoto, L. J.

AU - Anderson, R. S.

AU - Shearer, T. R.

N1 - Funding Information: ACKN3LEDGEMENTS This study was partially supported by NIH grants EY-03600 and EY-03856 to TRS and NIH grant EY-02932 to WT. The authors wish to thank Michael D. Varnum for expert technical assistance.

PY - 1988

Y1 - 1988

N2 - Experimental nuclear cataract produced by an overdose of sodium selenite exhibited limited proteolysis, including breakdown of main intrinsic polypeptide (MIP26) to 24 and 22 kD fragments (1). Micro-sequencing and site specific immunologic probes were used in the present study to determine regions of cleavage in MIP26 during selenite cataractogenesis. Data suggested that proteolysis occurred in the C-terminus of MIP26. This may have lead to exposure of normally hidden amino acid residues on the C-terminal extension of MIP26. Loss of antigenicity of the N-terminus occurred. These significant changes to the MIP26 molecule might adversely affect communication between lens fiber cells and contribute to selenite cataract.

AB - Experimental nuclear cataract produced by an overdose of sodium selenite exhibited limited proteolysis, including breakdown of main intrinsic polypeptide (MIP26) to 24 and 22 kD fragments (1). Micro-sequencing and site specific immunologic probes were used in the present study to determine regions of cleavage in MIP26 during selenite cataractogenesis. Data suggested that proteolysis occurred in the C-terminus of MIP26. This may have lead to exposure of normally hidden amino acid residues on the C-terminal extension of MIP26. Loss of antigenicity of the N-terminus occurred. These significant changes to the MIP26 molecule might adversely affect communication between lens fiber cells and contribute to selenite cataract.

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Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract

Abstract

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