TY - JOUR
T1 - Quantification of caffeoylquinic acids and triterpenes as targeted bioactive compounds of Centella Asiatica in extracts and formulations by liquid chromatography mass spectrometry
AU - Yang, Liping
AU - Marney, Luke
AU - Magana, Armando Alcazar
AU - Choi, Jaewoo
AU - Wright, Kirsten
AU - Mcferrin, Janis
AU - Gray, Nora E.
AU - Soumyanath, Amala
AU - Stevens, Jan F.
AU - Maier, Claudia S.
N1 - Publisher Copyright:
© 2023
PY - 2023/11/27
Y1 - 2023/11/27
N2 - Centella asiatica (CA) is a culinary vegetable and well-known functional food that is widely used as a medicinal herb and dietary supplement. CA is rich in pentacyclic triterpenes (TTs), including asiaticoside (AS), madecassoside (MS) and the related aglycones asiatic acid (AA), madecassic acid (MA). Traditionally, TTs have been associated with the bioactivity and health promoting effect of CA. Recently, mono-caffeoylquinic acids (MonoCQAs) and di-caffeoylquinic acids (DiCQAs) have been found to contribute to the bioactivity of CA as well. This work reports an analytical strategy based on liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM–MS) for the simultaneous rapid and accurate quantification of 12 bioactive compounds in CA, namely AS, MS, AA, MA, 5-CQA, 4-CQA, 3-CQA, 1,3-DiCQA, 3,4-DiCQA, 1,5-DiCQA, 3,5-DiCQA, 4,5-DiCQA. Method selectivity, accuracy, precision, repeatability, robustness, linearity range, limit of detection (LOD), and limit of quantitation (LOQ) were validated. The validated LC-MRM-MS method has been successfully applied to quantify the 12 bioactive compounds in CA aqueous extracts and two related formulations: a standardized CA product (CAP) used in a phase I clinical trial and formulated CA rodent diets used in preclinical studies. The validated method allows us to support the standardization of CA products used for clinical trials and conduct routine LC-MRM-MS analyses of formulated preclinical diets to confirm correct levels of CA phytochemical markers.
AB - Centella asiatica (CA) is a culinary vegetable and well-known functional food that is widely used as a medicinal herb and dietary supplement. CA is rich in pentacyclic triterpenes (TTs), including asiaticoside (AS), madecassoside (MS) and the related aglycones asiatic acid (AA), madecassic acid (MA). Traditionally, TTs have been associated with the bioactivity and health promoting effect of CA. Recently, mono-caffeoylquinic acids (MonoCQAs) and di-caffeoylquinic acids (DiCQAs) have been found to contribute to the bioactivity of CA as well. This work reports an analytical strategy based on liquid chromatography coupled to multiple reaction monitoring mass spectrometry (LC-MRM–MS) for the simultaneous rapid and accurate quantification of 12 bioactive compounds in CA, namely AS, MS, AA, MA, 5-CQA, 4-CQA, 3-CQA, 1,3-DiCQA, 3,4-DiCQA, 1,5-DiCQA, 3,5-DiCQA, 4,5-DiCQA. Method selectivity, accuracy, precision, repeatability, robustness, linearity range, limit of detection (LOD), and limit of quantitation (LOQ) were validated. The validated LC-MRM-MS method has been successfully applied to quantify the 12 bioactive compounds in CA aqueous extracts and two related formulations: a standardized CA product (CAP) used in a phase I clinical trial and formulated CA rodent diets used in preclinical studies. The validated method allows us to support the standardization of CA products used for clinical trials and conduct routine LC-MRM-MS analyses of formulated preclinical diets to confirm correct levels of CA phytochemical markers.
KW - Caffeoylquinic acids
KW - Centella asiatica
KW - Formulations
KW - LC-MRM-MS
KW - Triterpenes
UR - http://www.scopus.com/inward/record.url?scp=85165688655&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85165688655&partnerID=8YFLogxK
U2 - 10.1016/j.jcoa.2023.100091
DO - 10.1016/j.jcoa.2023.100091
M3 - Article
AN - SCOPUS:85165688655
SN - 2772-3917
VL - 4
JO - Journal of Chromatography Open
JF - Journal of Chromatography Open
M1 - 100091
ER -