Radiofrequency ablation lesions in a pig liver model

Paul D. Hansen, Stan Rogers, Christopher L. Corless, Lee L. Swanstrom, Allan E. Siperstien

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


Background. Radiofrequency (RF) ablation has been reported as a means of liver tumor destruction. This study evaluates the use of ultrasound monitoring of radiofrequency lesion creation and describes the morphology, histologic characteristics, and vascular effects of radiofrequency ablations in a pig liver model. Materials and methods, Hemodynamic monitoring was established and laparotomies were performed in 50-kg pigs. Under ultrasound guidance, radiofrequency needle probes were placed in the liver at predetermined locations. Radiofrequency energy was applied over 15 min to generate lesions 3 cm in diameter. Eighty lesions were generated in 10 animals. At the completion of the experiment, the lesions were examined with ultrasound and then excised for CT, gross, and histologic examination. Results. There were no adverse systemic effects. Ultrasound imaging demonstrated the size, shape, and position of the lesions. Gross examination demonstrated a core of ablated tissue with a surrounding 1 to 2-mm hemorrhagic perimeter. Lesion volumes averaged 12.8 cc3 (range 5-34 cc3). Final lesion shape and size were frequently altered by the cooling effect of local blood flow. Histologic stains demonstrated microvascular thrombosis and coagulative necrosis within the lesions. There appeared to be 100% cellular destruction within the lesion by cytochemical staining. Conclusions. We demonstrated that RF ablation is capable of killing large volumes of normal liver tissue; however, local vasculature plays a significant a role in defining the ultimate size and shape of the lesion created. This may interfere with the utility of radiofrequency ablation as a modality for local tumor control.

Original languageEnglish (US)
Pages (from-to)114-121
Number of pages8
JournalJournal of Surgical Research
Issue number1
StatePublished - Nov 1999


  • Ablation
  • Cancer
  • Hepatic
  • Liver
  • RFA
  • Radiofrequency
  • Tumor

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'Radiofrequency ablation lesions in a pig liver model'. Together they form a unique fingerprint.

Cite this