TY - JOUR
T1 - Rapid and sensitive detection of nano-fluidically trapped protein biomarkers
AU - Shanmugam, Nandhinee Radha
AU - Selvam, Anjan Panneer
AU - Barrett, Thomas W.
AU - Kazmierczak, Steve
AU - Prasad, Shalini
N1 - Publisher Copyright:
Copyright © Materials Research Society 2014Â.
PY - 2014
Y1 - 2014
N2 - In this work, we demonstrate the label-free and ultrasensitive detection of troponin-T, cardiac biomarker using nanoporous membrane integrated on a microelectrode sensor platform. The nanoporous membrane allows for spatial confinement of the protein molecules. Antigen interaction with thiol immobilized antibody perturbs the electrical double layer. Charge perturbations are recorded as impedance change at low frequency using the principle of electrochemical impedance spectroscopy (EIS). The measured impedance change is used to quantitatively determine the concentration of troponin-T in tested sample. We have shown that sensitivity of sensor for troponin-T to be 1pg/mL. The accuracy and reliability of this sensor was tested by comparing the experimental troponin-T concentration values with a commercially available electrochemiluminescence assay measured with Roche Elecsys analyzer. Using this technique we were successful in detecting protein biomarkers in whole blood, human serum, and ionic buffers. This technology provides a robust analytical platform for rapid and sensitive detection of protein biomarkers, thus establishing this technology as an ideal candidate for biomarker screening in clinical settings.
AB - In this work, we demonstrate the label-free and ultrasensitive detection of troponin-T, cardiac biomarker using nanoporous membrane integrated on a microelectrode sensor platform. The nanoporous membrane allows for spatial confinement of the protein molecules. Antigen interaction with thiol immobilized antibody perturbs the electrical double layer. Charge perturbations are recorded as impedance change at low frequency using the principle of electrochemical impedance spectroscopy (EIS). The measured impedance change is used to quantitatively determine the concentration of troponin-T in tested sample. We have shown that sensitivity of sensor for troponin-T to be 1pg/mL. The accuracy and reliability of this sensor was tested by comparing the experimental troponin-T concentration values with a commercially available electrochemiluminescence assay measured with Roche Elecsys analyzer. Using this technique we were successful in detecting protein biomarkers in whole blood, human serum, and ionic buffers. This technology provides a robust analytical platform for rapid and sensitive detection of protein biomarkers, thus establishing this technology as an ideal candidate for biomarker screening in clinical settings.
KW - nanoscale
KW - porosity
KW - sensor
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U2 - 10.1557/opl.2014.927
DO - 10.1557/opl.2014.927
M3 - Conference article
AN - SCOPUS:84924351342
SN - 0272-9172
VL - 1686
JO - Materials Research Society Symposium Proceedings
JF - Materials Research Society Symposium Proceedings
T2 - 2014 MRS Spring Meeting
Y2 - 21 April 2014 through 25 April 2014
ER -