TY - JOUR
T1 - Rapid iterative negative geotaxis (RING)
T2 - A new method for assessing age-related locomotor decline in Drosophila
AU - Gargano, Julia Warner
AU - Martin, Ian
AU - Bhandari, Poonam
AU - Grotewiel, Michael S.
N1 - Funding Information:
We thank Adam Goetschy (Michigan State University) for help with construction of the RING apparatus and Laurent Seroude (Queens University, Kingston, Ont., Canada) and Peter Foster-Fishman for helpful discussions. We also thank Ron Davis, Marc Tatar, Steve Helfand and the Bloomington Drosophila Stock Center at Indiana University for providing fly stocks. We are grateful for the expert technical assistance of William Horton, Melissa Borrusch and Jessica Howell. This work was supported by grants from the N.I.H. to M.S.G. (MH64160 and AG21199).
PY - 2005/5
Y1 - 2005/5
N2 - Age-related behavioral declines are common manifestations of aging in animals. Negative geotaxis, an innate escape response during which flies ascend the wall of a cylinder after being tapped to its bottom, is one of the behaviors that senesces in Drosophila. Many laboratories, including ours, have used a variety of negative geotaxis assays based on the performance of single flies. To circumvent limitations of single-fly assays, we developed a new method for assessing negative geotaxis called rapid iterative negative geotaxis (RING). In RING assays, digital photography is used to document negative geotaxis in multiple groups of animals simultaneously. We show that performance in RING assays is not influenced by the density of flies being tested, the time of day, or repeated testing. We used the RING assay to demonstrate that negative geotaxis declines with the age of animals as previously shown in single fly studies and that senescence of negative geotaxis is sensitive to genetic background. Finally, we used RING assays to show that long-lived Indy and chico mutants exhibit delayed senescence of negative geotaxis. Our results demonstrate that RING is a powerful method for assessing negative geotaxis that should facilitate the search for manipulations that influence behavioral aging in Drosophila.
AB - Age-related behavioral declines are common manifestations of aging in animals. Negative geotaxis, an innate escape response during which flies ascend the wall of a cylinder after being tapped to its bottom, is one of the behaviors that senesces in Drosophila. Many laboratories, including ours, have used a variety of negative geotaxis assays based on the performance of single flies. To circumvent limitations of single-fly assays, we developed a new method for assessing negative geotaxis called rapid iterative negative geotaxis (RING). In RING assays, digital photography is used to document negative geotaxis in multiple groups of animals simultaneously. We show that performance in RING assays is not influenced by the density of flies being tested, the time of day, or repeated testing. We used the RING assay to demonstrate that negative geotaxis declines with the age of animals as previously shown in single fly studies and that senescence of negative geotaxis is sensitive to genetic background. Finally, we used RING assays to show that long-lived Indy and chico mutants exhibit delayed senescence of negative geotaxis. Our results demonstrate that RING is a powerful method for assessing negative geotaxis that should facilitate the search for manipulations that influence behavioral aging in Drosophila.
KW - Aging
KW - Behavior
KW - Chico
KW - Fruit flies
KW - Functional senescence
KW - Indy
UR - http://www.scopus.com/inward/record.url?scp=20144366852&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20144366852&partnerID=8YFLogxK
U2 - 10.1016/j.exger.2005.02.005
DO - 10.1016/j.exger.2005.02.005
M3 - Article
C2 - 15919590
AN - SCOPUS:20144366852
SN - 0531-5565
VL - 40
SP - 386
EP - 395
JO - Experimental Gerontology
JF - Experimental Gerontology
IS - 5
ER -