TY - JOUR
T1 - Reactivation of latent human cytomegalovirus by allogeneic stimulation of blood cells from healthy donors
AU - Söderberg-Nauclér, Cecilia
AU - Fish, Kenneth N.
AU - Nelson, Jay A.
N1 - Funding Information:
We thank Nancy Peffer, David E. Michalik, Heather L. Meyers, Patsy Smith, and Justine Allan Yorke for technical assistance and Drs. Ashlee Moses, Magadeline So, Ann Hill, David Johnson, David Parker, and the members of the Nelson laboratory for helpful discussion. We also thank Andrew Townsend for his graphic work. This work was supported by a Public Health Service Grant from the National Institutes of Health AI 21640 (J. A. N.), the Molecular Hematology Training Program NIH NRSA Training Award (K. N. F.), and the Knut and Alice Wallenbergs Foundation (C. S.-N.). C. S. -N. is a scholar of the Wenner-Gren Foundation, Sweden.
PY - 1997/10/3
Y1 - 1997/10/3
N2 - Reactivation of human cytomegalovirus (HCMV) results in severe disease in AIDS patients and immunocompromised patients receiving blood transfusions or organ or bone marrow grafts. Although the site of HCMV latency is unknown, blood cells have been implicated as a viral reservoir. In this study, we demonstrate HCMV reactivation in vitro from seven consecutive healthy donors through allogeneic stimulation of peripheral blood mononuclear cells (PBMCs). HCMV replication was detected at 17 days poststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic cell markers. Thus, these observations demonstrate that PBMCs harbor latent HCMV, which reactivates in a myeloid lineage cell upon allogeneic stimulation.
AB - Reactivation of human cytomegalovirus (HCMV) results in severe disease in AIDS patients and immunocompromised patients receiving blood transfusions or organ or bone marrow grafts. Although the site of HCMV latency is unknown, blood cells have been implicated as a viral reservoir. In this study, we demonstrate HCMV reactivation in vitro from seven consecutive healthy donors through allogeneic stimulation of peripheral blood mononuclear cells (PBMCs). HCMV replication was detected at 17 days poststimulation, and virus was recovered after long-term culture from a macrophage expressing dendritic cell markers. Thus, these observations demonstrate that PBMCs harbor latent HCMV, which reactivates in a myeloid lineage cell upon allogeneic stimulation.
UR - http://www.scopus.com/inward/record.url?scp=0242421666&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242421666&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(01)80014-3
DO - 10.1016/S0092-8674(01)80014-3
M3 - Article
C2 - 9335340
AN - SCOPUS:0242421666
SN - 0092-8674
VL - 91
SP - 119
EP - 126
JO - Cell
JF - Cell
IS - 1
ER -