Real-time biosensor platform: Fully integrated device for impedimetric assays

Andrey L. Ghindilis, Kevin R. Schwarzkopf, Dean S. Messing, Ibrahim Sezan, Paul Schuele, Changqing Zhan, Maria W. Smith, Holly M. Simon, David R. Evans

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

An impedimetric biosensor platform for bio-affinity assays was developed based on real-time, label-free electrochemical detection performed via direct interface to electronic digital data processing. The sensor array consists of 15 gold microelectrode pairs enclosed in three reaction chambers and bio-functionalized with specific DNA probes. The impedance change caused by specific target analyte binding to the functionalized electrode surface is recorded in real time. The detector is capable of continuous and simultaneous stimulation and recording of all array electrodes. A corresponding mathematical algorithm, and a software package for data analysis were developed and used to quantify both the rate of target to probe binding, and target to probe affinity. The biosensor was capable of distinguishing between closely-related bacterial strains. This fully-integrated sensor array platform can be used for detection of a wide range of analytes of practical significance, and has potential for further integration with amplification (i.e. PCR) and sample preparation modules.

Original languageEnglish (US)
Title of host publicationChemical Sensors 9 -and- MEMS/NEMS 9
PublisherElectrochemical Society Inc.
Pages59-68
Number of pages10
Edition8
ISBN (Electronic)9781607681779
ISBN (Print)9781566778275
DOIs
StatePublished - 2010
Externally publishedYes

Publication series

NameECS Transactions
Number8
Volume33
ISSN (Print)1938-5862
ISSN (Electronic)1938-6737

ASJC Scopus subject areas

  • General Engineering

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