Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain

Sue A. Aicher; Mary Springston; Scott B. Berger; Donald J. Reis; Claes Wahlestedt

doi:10.1016/0304-3940(91)90220-N

Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain

Sue A. Aicher, Mary Springston, Scott B. Berger, Donald J. Reis, Claes Wahlestedt

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Neuropeptide Y (NPY) receptors are heterogeneous, consisting of at least two subclasses, Y₁ and Y₂. We sought evidence for differential expression of NPY receptor subtypes in the rat brain. Tissue was incubated with ¹²⁵I-peptide YY (PYY) which labels NPY and PYY binding sites. The Y₁-selective agonist, p[Pro³⁴]NPY, and the Y₂-selective agonist, pNPY 13-36, were used as displacing ligands. Autoradiographic analyses of regional receptor binding demonstrated heterogeneity across brain regions. We conclude that Y₁- and Y₂-receptors may be independently expressed in the brain. While the predominate NPY/PYY receptor subtype in the brain is Y₂, there are also Y₁-receptors in some brain regions such as the superficial layers of the parietal cortex.

Original language	English (US)
Pages (from-to)	32-36
Number of pages	5
Journal	Neuroscience Letters
Volume	130
Issue number	1
DOIs	https://doi.org/10.1016/0304-3940(91)90220-N
State	Published - Sep 2 1991
Externally published	Yes

Keywords

Neuropeptide Y
Neuropeptide Y analogs
Receptor
Receptor autoradiography

ASJC Scopus subject areas

General Neuroscience

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10.1016/0304-3940(91)90220-N

Cite this

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title = "Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain",

abstract = "Neuropeptide Y (NPY) receptors are heterogeneous, consisting of at least two subclasses, Y1 and Y2. We sought evidence for differential expression of NPY receptor subtypes in the rat brain. Tissue was incubated with 125I-peptide YY (PYY) which labels NPY and PYY binding sites. The Y1-selective agonist, p[Pro34]NPY, and the Y2-selective agonist, pNPY 13-36, were used as displacing ligands. Autoradiographic analyses of regional receptor binding demonstrated heterogeneity across brain regions. We conclude that Y1- and Y2-receptors may be independently expressed in the brain. While the predominate NPY/PYY receptor subtype in the brain is Y2, there are also Y1-receptors in some brain regions such as the superficial layers of the parietal cortex.",

keywords = "Neuropeptide Y, Neuropeptide Y analogs, Receptor, Receptor autoradiography",

author = "Aicher, {Sue A.} and Mary Springston and Berger, {Scott B.} and Reis, {Donald J.} and Claes Wahlestedt",

year = "1991",

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doi = "10.1016/0304-3940(91)90220-N",

language = "English (US)",

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pages = "32--36",

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T1 - Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain

AU - Aicher, Sue A.

AU - Springston, Mary

AU - Berger, Scott B.

AU - Reis, Donald J.

AU - Wahlestedt, Claes

PY - 1991/9/2

Y1 - 1991/9/2

N2 - Neuropeptide Y (NPY) receptors are heterogeneous, consisting of at least two subclasses, Y1 and Y2. We sought evidence for differential expression of NPY receptor subtypes in the rat brain. Tissue was incubated with 125I-peptide YY (PYY) which labels NPY and PYY binding sites. The Y1-selective agonist, p[Pro34]NPY, and the Y2-selective agonist, pNPY 13-36, were used as displacing ligands. Autoradiographic analyses of regional receptor binding demonstrated heterogeneity across brain regions. We conclude that Y1- and Y2-receptors may be independently expressed in the brain. While the predominate NPY/PYY receptor subtype in the brain is Y2, there are also Y1-receptors in some brain regions such as the superficial layers of the parietal cortex.

AB - Neuropeptide Y (NPY) receptors are heterogeneous, consisting of at least two subclasses, Y1 and Y2. We sought evidence for differential expression of NPY receptor subtypes in the rat brain. Tissue was incubated with 125I-peptide YY (PYY) which labels NPY and PYY binding sites. The Y1-selective agonist, p[Pro34]NPY, and the Y2-selective agonist, pNPY 13-36, were used as displacing ligands. Autoradiographic analyses of regional receptor binding demonstrated heterogeneity across brain regions. We conclude that Y1- and Y2-receptors may be independently expressed in the brain. While the predominate NPY/PYY receptor subtype in the brain is Y2, there are also Y1-receptors in some brain regions such as the superficial layers of the parietal cortex.

KW - Neuropeptide Y

KW - Neuropeptide Y analogs

KW - Receptor

KW - Receptor autoradiography

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JF - Neuroscience Letters

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ER -

Receptor-selective analogs demonstrate NPY/PYY receptor heterogeneity in rat brain

Abstract

Keywords

ASJC Scopus subject areas

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