TY - JOUR
T1 - Recovery from an Acute Infection in C. elegans Requires the GATA Transcription Factor ELT-2
AU - Head, Brian
AU - Aballay, Alejandro
N1 - Funding Information:
C. elegans strains were provided by the Caenorhabditis Genetics Center (University of Minnesota), which is funded by the NIH-ORIP. We thank the Duke IGSP Microarray Core Facility for technical advice.
Publisher Copyright:
© 2014 Head, Aballay.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - The mechanisms involved in the recognition of microbial pathogens and activation of the immune system have been extensively studied. However, the mechanisms involved in the recovery phase of an infection are incompletely characterized at both the cellular and physiological levels. Here, we establish a Caenorhabditis elegans-Salmonella enterica model of acute infection and antibiotic treatment for studying biological changes during the resolution phase of an infection. Using whole genome expression profiles of acutely infected animals, we found that genes that are markers of innate immunity are down-regulated upon recovery, while genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis are up-regulated. In silico analyses demonstrated that genes altered during recovery from infection were transcriptionally regulated by conserved transcription factors, including GATA/ELT-2, FOXO/DAF-16, and Nrf/SKN-1. Finally, we found that recovery from an acute bacterial infection is dependent on ELT-2 activity.
AB - The mechanisms involved in the recognition of microbial pathogens and activation of the immune system have been extensively studied. However, the mechanisms involved in the recovery phase of an infection are incompletely characterized at both the cellular and physiological levels. Here, we establish a Caenorhabditis elegans-Salmonella enterica model of acute infection and antibiotic treatment for studying biological changes during the resolution phase of an infection. Using whole genome expression profiles of acutely infected animals, we found that genes that are markers of innate immunity are down-regulated upon recovery, while genes involved in xenobiotic detoxification, redox regulation, and cellular homeostasis are up-regulated. In silico analyses demonstrated that genes altered during recovery from infection were transcriptionally regulated by conserved transcription factors, including GATA/ELT-2, FOXO/DAF-16, and Nrf/SKN-1. Finally, we found that recovery from an acute bacterial infection is dependent on ELT-2 activity.
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U2 - 10.1371/journal.pgen.1004609
DO - 10.1371/journal.pgen.1004609
M3 - Article
C2 - 25340560
AN - SCOPUS:84908331625
SN - 1553-7390
VL - 10
JO - PLoS genetics
JF - PLoS genetics
IS - 10
ER -