Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

M. B. Lewin; A. Wong; C. W. Deveney; H. Sankaran

doi:10.1016/0014-5793(85)80618-9

Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

M. B. Lewin, A. Wong, C. W. Deveney, H. Sankaran

Research output: Contribution to journal › Article › peer-review

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Abstract

The Ca²⁺ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

Original language	English (US)
Pages (from-to)	259-262
Number of pages	4
Journal	FEBS Letters
Volume	184
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(85)80618-9
State	Published - May 20 1985
Externally published	Yes

Keywords

Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(85)80618-9

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title = "Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini",

abstract = "The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.",

keywords = "Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility",

author = "Lewin, {M. B.} and A. Wong and Deveney, {C. W.} and H. Sankaran",

note = "Funding Information: We thank Mr James R. Gorring for his excellent secretariala ssistanceS. upported by grants from the VeteransA dministrationa nd MS281 from the University of California, San Francisco, CA.",

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doi = "10.1016/0014-5793(85)80618-9",

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T1 - Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

AU - Lewin, M. B.

AU - Wong, A.

AU - Deveney, C. W.

AU - Sankaran, H.

N1 - Funding Information: We thank Mr James R. Gorring for his excellent secretariala ssistanceS. upported by grants from the VeteransA dministrationa nd MS281 from the University of California, San Francisco, CA.

PY - 1985/5/20

Y1 - 1985/5/20

N2 - The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

AB - The Ca2+ ionophore, A23187, stimulated amylase secretion from isolated rat pancreatic acini in a dosedependent manner with a maximal effect at 6 μM. Acetaldehyde, a metabolite of ethanol, caused a reduction in the magnitude of ionophore-stimulated secretion with no evidence of competitive inhibition. Furthermore, 6 μM ionophore-stimulated amylase secretion was dose-dependently inhibited by acetaldehyde. This inhibitory effect of acetaldehyde, however, was reversible on washing and reincubating acetaldehyde-treated acini. These results suggest that acetaldehyde reversibly inhibits intracellular components mediating stimulated secretion and this inhibition requires a continuous chemical interaction between acetaldehyde and intracellular component(s) regulating stimulated enzyme secretion.

KW - Acetaldehyde Pancreatic acini Calcium ionophore Amylase secretion Reversibility

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Reversible acetaldehyde inhibition of A23187-stimulated amylase secretion from isolated rat pancreatic acini

Abstract

Keywords

ASJC Scopus subject areas

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