Role of cycle threshold in clostridioides difficile polymerase chain reaction testing as a predictor of clinical outcomes in patients with inflammatory bowel disease

Emily R. Jonica; Carol A. Sulis; Kanupriya Soni; Michelle Hughes; Eric Jones; Janice Weinberg; Nancy S. Miller; Francis A. Farraye

doi:10.1093/crocol/otz036

Role of cycle threshold in clostridioides difficile polymerase chain reaction testing as a predictor of clinical outcomes in patients with inflammatory bowel disease

Emily R. Jonica, Carol A. Sulis, Kanupriya Soni, Michelle Hughes, Eric Jones, Janice Weinberg, Nancy S. Miller, Francis A. Farraye

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: Distinguishing Clostridioides difficile infection from colonization is challenging in patients with Inflammatory Bowel Disease (IBD). Cycle threshold (Ct), the cutoff for PCR positivity, has been investigated in non-IBD patients. Methods: Patients with positive C. difficile PCR (25 IBD, 51 non-IBD) were identified retrospectively. Fifteen-day outcomes were assessed. Results: Ct correlated with diarrheal days in non-IBD (P = 0.048), but not IBD patients (P = 0.769). IBD patients had shorter LOS and less severe infection, but more diarrheal days (P < 0.05). Conclusions: IBD patients had a milder course but Ct results were not significant. Larger studies are needed to clarify utility of Ct in IBD.

Original language	English (US)
Journal	Crohn's and Colitis 360
Volume	1
Issue number	3
DOIs	https://doi.org/10.1093/crocol/otz036
State	Published - Oct 1 2019
Externally published	Yes

Keywords

Clostridium difficile
Cycle threshold
Inflammatory bowel disease

ASJC Scopus subject areas

Gastroenterology

Access to Document

10.1093/crocol/otz036

Cite this

@article{48e87ac5101441729f33c4bb167a5336,

title = "Role of cycle threshold in clostridioides difficile polymerase chain reaction testing as a predictor of clinical outcomes in patients with inflammatory bowel disease",

abstract = "Background: Distinguishing Clostridioides difficile infection from colonization is challenging in patients with Inflammatory Bowel Disease (IBD). Cycle threshold (Ct), the cutoff for PCR positivity, has been investigated in non-IBD patients. Methods: Patients with positive C. difficile PCR (25 IBD, 51 non-IBD) were identified retrospectively. Fifteen-day outcomes were assessed. Results: Ct correlated with diarrheal days in non-IBD (P = 0.048), but not IBD patients (P = 0.769). IBD patients had shorter LOS and less severe infection, but more diarrheal days (P < 0.05). Conclusions: IBD patients had a milder course but Ct results were not significant. Larger studies are needed to clarify utility of Ct in IBD.",

keywords = "Clostridium difficile, Cycle threshold, Inflammatory bowel disease",

author = "Jonica, {Emily R.} and Sulis, {Carol A.} and Kanupriya Soni and Michelle Hughes and Eric Jones and Janice Weinberg and Miller, {Nancy S.} and Farraye, {Francis A.}",

note = "Publisher Copyright: {\textcopyright} 2019 Crohn's & Colitis Foundation. Published by Oxford University Press on behalf of Crohn's & Colitis Foundation.",

year = "2019",

month = oct,

day = "1",

doi = "10.1093/crocol/otz036",

language = "English (US)",

volume = "1",

journal = "Crohn's and Colitis 360",

issn = "2631-827X",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Role of cycle threshold in clostridioides difficile polymerase chain reaction testing as a predictor of clinical outcomes in patients with inflammatory bowel disease

AU - Jonica, Emily R.

AU - Sulis, Carol A.

AU - Soni, Kanupriya

AU - Hughes, Michelle

AU - Jones, Eric

AU - Weinberg, Janice

AU - Miller, Nancy S.

AU - Farraye, Francis A.

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Background: Distinguishing Clostridioides difficile infection from colonization is challenging in patients with Inflammatory Bowel Disease (IBD). Cycle threshold (Ct), the cutoff for PCR positivity, has been investigated in non-IBD patients. Methods: Patients with positive C. difficile PCR (25 IBD, 51 non-IBD) were identified retrospectively. Fifteen-day outcomes were assessed. Results: Ct correlated with diarrheal days in non-IBD (P = 0.048), but not IBD patients (P = 0.769). IBD patients had shorter LOS and less severe infection, but more diarrheal days (P < 0.05). Conclusions: IBD patients had a milder course but Ct results were not significant. Larger studies are needed to clarify utility of Ct in IBD.

AB - Background: Distinguishing Clostridioides difficile infection from colonization is challenging in patients with Inflammatory Bowel Disease (IBD). Cycle threshold (Ct), the cutoff for PCR positivity, has been investigated in non-IBD patients. Methods: Patients with positive C. difficile PCR (25 IBD, 51 non-IBD) were identified retrospectively. Fifteen-day outcomes were assessed. Results: Ct correlated with diarrheal days in non-IBD (P = 0.048), but not IBD patients (P = 0.769). IBD patients had shorter LOS and less severe infection, but more diarrheal days (P < 0.05). Conclusions: IBD patients had a milder course but Ct results were not significant. Larger studies are needed to clarify utility of Ct in IBD.

KW - Clostridium difficile

KW - Cycle threshold

KW - Inflammatory bowel disease

UR - http://www.scopus.com/inward/record.url?scp=85089450912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85089450912&partnerID=8YFLogxK

U2 - 10.1093/crocol/otz036

DO - 10.1093/crocol/otz036

M3 - Article

AN - SCOPUS:85089450912

SN - 2631-827X

VL - 1

JO - Crohn's and Colitis 360

JF - Crohn's and Colitis 360

IS - 3

ER -

Role of cycle threshold in clostridioides difficile polymerase chain reaction testing as a predictor of clinical outcomes in patients with inflammatory bowel disease

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this