Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons

Yan Na Wu; Giuseppina Martella; Steven W. Johnson

doi:10.1097/WNR.0b013e3282f0d28f

Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons

Yan Na Wu, Giuseppina Martella, Steven W. Johnson

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Our previous work showed that the pesticide rotenone increases the amplitude of inward currents evoked by N-methyl-D-aspartate (NMDA) in substantia nigra dopamine neurons. Using patch pipettes to record whole-cell currents in rat brain slices, we report that the rotenone-induced potentiation of NMDA current is blocked by the tyrosine kinase inhibitors genistein and PP1. This action of rotenone is mimicked by H2O2, which is also blocked by genistein. Our results suggest that the rotenone-dependent increase in NMDA current is mediated by release of reactive oxygen species that activates a protein tyrosine kinase.

Original language	English (US)
Pages (from-to)	1813-1816
Number of pages	4
Journal	NeuroReport
Volume	18
Issue number	17
DOIs	https://doi.org/10.1097/WNR.0b013e3282f0d28f
State	Published - Nov 2007
Externally published	Yes

Keywords

Brain slice
Excitotoxicity
Hydrogen peroxide
N-methyl-D-aspartate
Patch clamp
Reactive oxygen species
Rotenone
Substantia nigra
Tyrosine kinase

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1097/WNR.0b013e3282f0d28f

Cite this

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title = "Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons",

abstract = "Our previous work showed that the pesticide rotenone increases the amplitude of inward currents evoked by N-methyl-D-aspartate (NMDA) in substantia nigra dopamine neurons. Using patch pipettes to record whole-cell currents in rat brain slices, we report that the rotenone-induced potentiation of NMDA current is blocked by the tyrosine kinase inhibitors genistein and PP1. This action of rotenone is mimicked by H2O2, which is also blocked by genistein. Our results suggest that the rotenone-dependent increase in NMDA current is mediated by release of reactive oxygen species that activates a protein tyrosine kinase.",

keywords = "Brain slice, Excitotoxicity, Hydrogen peroxide, N-methyl-D-aspartate, Patch clamp, Reactive oxygen species, Rotenone, Substantia nigra, Tyrosine kinase",

author = "Wu, {Yan Na} and Giuseppina Martella and Johnson, {Steven W.}",

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language = "English (US)",

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T1 - Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons

AU - Wu, Yan Na

AU - Martella, Giuseppina

AU - Johnson, Steven W.

PY - 2007/11

Y1 - 2007/11

N2 - Our previous work showed that the pesticide rotenone increases the amplitude of inward currents evoked by N-methyl-D-aspartate (NMDA) in substantia nigra dopamine neurons. Using patch pipettes to record whole-cell currents in rat brain slices, we report that the rotenone-induced potentiation of NMDA current is blocked by the tyrosine kinase inhibitors genistein and PP1. This action of rotenone is mimicked by H2O2, which is also blocked by genistein. Our results suggest that the rotenone-dependent increase in NMDA current is mediated by release of reactive oxygen species that activates a protein tyrosine kinase.

AB - Our previous work showed that the pesticide rotenone increases the amplitude of inward currents evoked by N-methyl-D-aspartate (NMDA) in substantia nigra dopamine neurons. Using patch pipettes to record whole-cell currents in rat brain slices, we report that the rotenone-induced potentiation of NMDA current is blocked by the tyrosine kinase inhibitors genistein and PP1. This action of rotenone is mimicked by H2O2, which is also blocked by genistein. Our results suggest that the rotenone-dependent increase in NMDA current is mediated by release of reactive oxygen species that activates a protein tyrosine kinase.

KW - Brain slice

KW - Excitotoxicity

KW - Hydrogen peroxide

KW - N-methyl-D-aspartate

KW - Patch clamp

KW - Reactive oxygen species

KW - Rotenone

KW - Substantia nigra

KW - Tyrosine kinase

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ER -

Rotenone enhances N-methyl-D-aspartate currents by activating a tyrosine kinase in rat dopamine neurons

Abstract

Keywords

ASJC Scopus subject areas

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