@article{67e38884a66a46c284f871fb08604a37,
title = "Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo",
abstract = "Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of embryonic HSCs and contribute new insight into their cellular origin.",
author = "North, {Trista E.} and {De Bruijn}, {Marella F.T.R.} and Terryl Stacy and Laleh Talebian and Evan Lind and Catherine Robin and Michael Binder and Elaine Dzierzak and Speck, {Nancy A.}",
note = "Funding Information: We thank Alice Given, Gary Ward, Corn{\'e} Snoys, and Steve Fiering for their technical advice and Gus Lienhard for the Irs3 −/− mice. T.E.N. was supported by T32GM08704 from the NIH/GM. N.A.S. is supported by Public Health Service grant R01CA58343, and E.D. by R01DK054077. M.F.T.R.d.B. is supported by a fellowship from the Dutch Cancer Society, and C.R. by La Ligue Nationale Contre le Cancer. Flow cytometry at Dartmouth Medical School was done in The Herbert C. Englert Cell Analysis Laboratory, established by a grant from the Fannie E. Rippel Foundation and supported in part by the Core Grant of the Norris Cotton Cancer Center (CA 23108).",
year = "2002",
doi = "10.1016/S1074-7613(02)00296-0",
language = "English (US)",
volume = "16",
pages = "661--672",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "5",
}