Serum antibodies against βH-crystallins in the American Cocker Spaniel

Nobuyuki Kanemaki, Chiho Fukiage, Yoichiro Ichikawa, Thomas R. Shearer, Mitsuyoshi Azuma

Research output: Contribution to journalArticlepeer-review


Objective: To detect antibodies for lens βH-crystallins in the serum from the American Cocker Spaniel (ACS) presenting with and without cataracts and with and without uveitis. Animal Studied: Seventy-three American Cocker Spaniels and six normal Beagles. Procedures: Sera were collected from 73 ACSs, including those with normal lenses and those with cataracts, or uveitis. Fractionated, normal Beagle lens βH-crystallins were separated by one- or two-dimensional electrophoresis. The separated lens βH-crystallins were used on immunoblots as sentinel substrates against which the ACS sera were tested for the presence of antibodies against βH-crystallins. Results: Sera from approximately two-thirds of study animals contained antibodies to some βH-crystallin polypeptides, but reactivity varied among patients. Contrary to some hypotheses, serum antibodies to groups of βH-crystallins did not relate to the stages of cataract. However, detailed analysis by two-dimensional immunoblotting and mass spectrometry showed that three spots originating from βA1-crystallin were detected only in sera from cataract patients. Conclusion: Serum antibodies to βA1-crystallin may be associated with the development of cataract.

Original languageEnglish (US)
Pages (from-to)109-115
Number of pages7
JournalVeterinary Ophthalmology
Issue number2
StatePublished - Mar 1 2015
Externally publishedYes


  • AmericanCockerSpaniels
  • Cataracts
  • Dog
  • Immunoblotting
  • Serum antibodies
  • βH-crystallins

ASJC Scopus subject areas

  • General Veterinary


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