@inbook{3eb62cec2f6f40c4ac9b7b46d3571b26,
title = "Simple and effective affinity purification procedures for mass spectrometry-based identification of protein-protein interactions in cell signaling pathways",
abstract = "Identification of protein-protein interactions can be a critical step in understanding the function and regulation of a particular protein and for exploring intracellular signaling cascades. Affinity purification coupled to mass spectrometry (APMS) is a powerful method for isolating and characterizing protein complexes. This approach involves the tagging and subsequent enrichment of a protein of interest along with any stably associated proteins that bind to it, followed by the identification of the interacting proteins using mass spectrometry. The protocol described here offers a quick and simple method for routine sample preparation for APMS analysis of suitably tagged human cell lines.",
keywords = "Affinity purification-mass spectrometry, Cell signaling pathway, Liquid chromatography-mass spectrometry",
author = "Kwan, {Julian H.M.} and Andrew Emili",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-3341-9_13",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "181--187",
booktitle = "Methods in Molecular Biology",
}